Oxidized Alginate-Gelatin Hydrogel: A Favorable Matrix for Growth and Osteogenic Differentiation of Adipose-Derived Stem Cells in 3D,ACS Biomaterials Science & Engineering

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Oxidized Alginate-Gelatin Hydrogel: A Favorable Matrix for Growth and Osteogenic Differentiation of Adipose-Derived Stem Cells in 3D
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2017-07-07 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00188
Bapi Sarker ₁ , Tobias Zehnder ₁ , Subha N. Rath ₂ , Raymund E. Horch ₂ , Ulrich Kneser ₃ , Rainer Detsch ₁ , Aldo R. Boccaccini ₁

Affiliation

Alginate-based hydrogels are extensively used matrices for cell encapsulation, but they need to be modified to recapitulate chemical, microstructural, and mechanical properties of the native extracellular matrix. Like other cell types, mesenchymal stem cells exhibit rounded and clustered morphologies when they are embedded in alginate hydrogels. In this study, we use covalently cross-linked oxidized alginate-gelatin hydrogels to encapsulate human adipose-derived stem cells in order to investigate cell growth, viability, and morphology during osteogenic differentiation taking advantage of the different physicochemical properties of this modified alginate-based hydrogel in comparison to those of the pristine alginate hydrogel. We investigate the effect of hydrogel compositions on stem cell behavior in 3D. Higher viability and the spreading morphology of encapsulated cells with interconnected networks were observed in high gelatin containing compositions. More filopodial protrusions from multicellular nodules were noticed during osteogenic differentiation in the hydrogels having a high amount of gelatin, confirming their suitability for cell encapsulation and bone tissue engineering applications.

中文翻译：

氧化的藻酸盐-明胶水凝胶：脂肪干细胞在3D中生长和成骨分化的有利基质

基于藻酸盐的水凝胶是细胞封装中广泛使用的基质，但是需要对其进行修饰以概括天然细胞外基质的化学，微观结构和机械性质。与其他细胞类型一样，间充质干细胞嵌入藻酸盐水凝胶后会呈现圆形和簇状形态。在这项研究中，我们使用共价交联的氧化藻酸盐-明胶水凝胶封装人脂肪来源的干细胞，以便利用这种基于藻酸盐的修饰物的不同理化特性来研究成骨分化过程中的细胞生长，活力和形态。与原始藻酸盐水凝胶相比。我们研究了水凝胶成分对3D干细胞行为的影响。在含有高明胶的组合物中观察到具有互连网络的封装细胞具有更高的生存力和分布形态。在具有大量明胶的水凝胶的成骨分化过程中，发现了多细胞结节的更多丝状突起，这证实了它们适用于细胞封装和骨组织工程应用。

更新日期：2017-07-07

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