Two novel 16-membered tetraene marcolides, bafilomycins N (1) and O (2), along with two known analogues, JBIR-100 (3) and bafilomycin K (4) were produced from isolate GIC10-1, a Streptomyces sp. strain that was originally cloned from bacterial communities associated with marine sponge Theonella sp. The structures of new bafilomycins 1 and 2 were established by using spectroscopic methods and comparing the spectroscopic data with those of known related metabolites. Compounds 1 and 2 were proven to be the first 14-methylbafilomycins to be isolated. Cytotoxicity of these compounds toward various cancer cell lines also is described.

从分离株GIC10-1（链霉菌属）中产生了两个新颖的16元四烯变质杆菌，bafilomycins N（1）和O（2）以及两种已知类似物JBIR-100（3）和bafilomycin K（4）。菌株最初是从与海洋海绵Theonella sp。相关的细菌群落中克隆的。通过使用光谱学方法，并将光谱数据与已知相关代谢物的光谱数据进行比较，来建立新的bafilomycins 1和2的结构。化合物1和2被证明是第一个被分离的14-甲基bafilomycins。还描述了这些化合物对各种癌细胞系的细胞毒性。