First Identification of Palytoxin-Like Molecules in the Atlantic Coral Species Palythoa canariensis,Analytical Chemistry

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First Identification of Palytoxin-Like Molecules in the Atlantic Coral Species Palythoa canariensis
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-07-07 00:00:00 , DOI: 10.1021/acs.analchem.7b01003
María Fraga ₁ , Natalia Vilariño ₁ , M. Carmen Louzao ₁ , Lucía Molina ₂ , Yanira López ₂ , Mark Poli ₃ , Luis M. Botana ₁

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Palytoxin (PLTX) is a complex marine toxin produced by Zoanthids (Palyhtoa), dinoflagellates (Ostreopsis), and cyanobacteria (Trichodesmium). Contact with PLTX-like compounds present in aerosols or marine organisms has been associated with adverse effects on humans. The worldwide distribution of producer species and seafood contaminated with PLTX-like molecules illustrates the global threat to human health. The identification of species capable of palytoxin production is critical for human safety. We studied the presence of PLTX analogues in Palythoa canariensis, a coral species collected in the Atlantic Ocean never described as a PLTX-producer before. Two methodologies were used for the detection of these toxins: a microsphere-based immunoassay that offered an estimation of the content of PLTX-like molecules in a Palythoa canariensis extract and an ultrahigh-pressure liquid chromatography coupled to an ion trap with a time-of-flight mass spectrometer (UPLC-IT-TOF-MS) that allowed the characterization of the toxin profile. The results demonstrated the presence of PLTX, hydroxy-PLTX and, at least, two additional compounds with PLTX-like profile in the Palythoa canariensis sample. The PLTX content was estimated in 0.27 mg/g of lyophilized coral using UPLC-IT-TOF-MS. Therefore, this work demonstrates that Palythoa canariensis produces a mixture of PLTX-like molecules. This is of special relevance to safeguard human health considering Palythoa species are commonly used for decoration by aquarium hobbyists.

中文翻译：

大西洋珊瑚物种Palythoa canariensis中类似于Pa毒素的分子的首次鉴定

Palytoxin（PLTX）是复杂的海洋毒素，由Zoanthids（Palyhtoa），鞭毛藻（Ostreopsis）和蓝细菌（Trichodesmium）产生。与气溶胶或海洋生物中存在的PLTX样化合物接触已对人类产生不利影响。生产者物种和受PLTX样分子污染的海产品在世界范围内的分布说明了对人类健康的全球威胁。鉴定能够产生palytoxin的物种对于人类安全至关重要。我们研究了加拿大柏树（Palythoa canariensis）中PLTX类似物的存在是在大西洋中收集的一种珊瑚，以前从未描述过PLTX的生产者。两种方法被用于检测这些毒素的：即提供的内容的估计的基于微球的免疫测定PLTX样在分子Palythoa海枣耦合到一个离子阱具有时间的提取物和超高压液相色谱飞行质谱仪（UPLC-IT-TOF-MS），可以表征毒素谱。结果表明在加拿大柏树（Palythoa canariensis）样品中存在PLTX，羟基-PLTX和至少两种其他具有PLTX样特征的化合物。使用UPLC-IT-TOF-MS估算PLTX含量为0.27 mg / g冻干珊瑚。因此，这项工作证明了加拿大柏树产生PLTX样分子的混合物。考虑到鱼鳞爱好者通常将水藻属物种用于装饰，这对维护人类健康特别重要。

更新日期：2017-07-08

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