A novel biphenylneolignan, 2,6,2',6'-tetramethoxy-4,4'-bis(2,3-epoxy-1-hydroxypropyl)biphenyl (1), and two new glycosides named atratoglaucosides A (2) and B (3), were isolated from the roots of Cynanchum atratum, and their structures were determined on the basis of chemical and spectroscopic evidence. The aglycons of 2 and 3 were identified as glaucogenin C and 7-desoxyneocynapanogenin A, a new disecopregnane. A known compound, glaucogenin C 3-O-beta-D-cymaropyranosyl-(1-->4)-alpha-L-diginopyranosyl-(1-->4)-beta-D-theveto pyranoside (4), isolated from the same source, showed a significant cytotoxic effect against 212 cells. This substance also gave a significant inhibitory effect on TNF-alpha (tumor necrosis factor-alpha) formation from the RAW 264.7 mouse macrophage-like cell line stimulated with LPS (lipopolysaccharide) and on the N9 microglial cell line stimulated with LPS/IFN-gamma (interferon-gamma).

中文翻译：

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牛头草根的生物活性成分。

一种新型的联苯新木聚糖，2,6,2'，6'-四甲氧基-4,4'-双（2,3-环氧-1-羟丙基）联苯（1）和两个新的糖苷分别命名为atratoglaucosides A（2）和B （3）是从白头翁根中分离出来的，其结构是根据化学和光谱学证据确定的。2和3的糖苷配基被鉴定为新的二癸红素葡聚糖原C和7-脱氧新烟碱泛素A。已知的化合物，青霉素C3-O-β-D-吡喃并吡喃糖基-（1→4）-α-L-地平吡喃糖基-（1→4）-β-D-巯基吡喃糖苷（4），分离自同一来源显示出对212个细胞的明显细胞毒性作用。该物质还对RAW 264形成的TNF-α（肿瘤坏死因子-α）产生了明显的抑制作用。