DNA Condensation Induced by a Star-Shaped Hexameric Cationic Surfactant,ACS Applied Materials & Interfaces

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DNA Condensation Induced by a Star-Shaped Hexameric Cationic Surfactant
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2017-07-03 00:00:00 , DOI: 10.1021/acsami.7b04317
Yaxun Fan , Hua Wang ₁ , Chengqian He , Fulin Qiao ₁ , Shu Wang , Yilin Wang ₁

Affiliation

The interactions between a star-shaped hexameric cationic quaternary ammonium surfactant PAHB and calf thymus DNA and induced DNA condensation were investigated by ζ-potential, dynamic light scattering, atomic force microscopy, isothermal titration calorimetry, ethidium bromide exclusion assay, circular dichroism, and cytotoxicity assay. With the addition of PAHB, long extended DNA molecules exhibit successive conformational transitions from elongated coil to a partially condensed cluster-like aggregate, to a globules-on-a-string structure, and then to a fully condensed globule until the saturation point of interaction between PAHB and DNA, which is slightly above their charge neutralization point. The efficient condensation is mainly produced by the strong attractive electrostatic interaction between the multiple positively charged headgroups of PAHB and negatively charged phosphate groups of DNA, and the hydrophobic interaction among the multiple alkyl chains of PAHB. Moreover the transition of the DNA conformation is also affected by the transitions of PAHB molecular conformation from star-shaped to claw-like and pyramid-like. Although the DNA conformation is significantly changed by PAHB, the DNA secondary structure does not display obvious variations, and the PAHB/DNA mixture does not show cytotoxicity when DNA is partially condensed. These results indicate that star-shaped oligomeric cationic surfactant is a potential condensing agent for gene transfection.

中文翻译：

星形六聚体阳离子表面活性剂诱导的DNA缩合

通过ζ电位，动态光散射，原子力显微镜，等温滴定量热法，溴化乙锭排除法，圆二色性和细胞毒性，研究了星形六聚阳离子阳离子季铵盐表面活性剂PAHB与小牛胸腺DNA之间的相互作用以及诱导的DNA缩合。分析。通过添加PAHB，长的延伸DNA分子呈现出连续的构象转变，从细长的螺旋到部分浓缩的簇状聚集体，再到串珠状小球，再到完全浓缩的小球，直至相互作用的饱和点在PAHB和DNA之间，略高于其电荷中和点。有效的缩合主要是通过PAHB的多个带正电荷的头基与DNA的带负电荷的磷酸基团之间的强吸引力静电相互作用，以及PAHB的多个烷基链之间的疏水相互作用而产生的。此外，DNA构象的转变也受到PAHB分子构象从星形到爪形和金字塔形的转变的影响。尽管PAHB显着改变了DNA构象，但DNA二级结构没有显示出明显的变化，并且当DNA部分缩合时，PAHB / DNA混合物也没有细胞毒性。这些结果表明星形低聚阳离子表面活性剂是基因转染的潜在缩合剂。以及PAHB多个烷基链之间的疏水相互作用。此外，DNA构象的转变也受到PAHB分子构象从星形到爪形和金字塔形的转变的影响。尽管PAHB显着改变了DNA构象，但DNA二级结构没有显示出明显的变化，并且当DNA部分缩合时，PAHB / DNA混合物没有细胞毒性。这些结果表明星形低聚阳离子表面活性剂是基因转染的潜在缩合剂。以及PAHB多个烷基链之间的疏水相互作用。此外，DNA构象的转变也受到PAHB分子构象从星形到爪形和金字塔形的转变的影响。尽管PAHB显着改变了DNA构象，但DNA二级结构没有显示出明显的变化，并且当DNA部分缩合时，PAHB / DNA混合物没有细胞毒性。这些结果表明星形低聚阳离子表面活性剂是基因转染的潜在缩合剂。DNA的二级结构没有明显的变化，并且DNA部分缩合时PAHB / DNA混合物没有细胞毒性。这些结果表明星形低聚阳离子表面活性剂是基因转染的潜在缩合剂。DNA的二级结构没有明显的变化，并且DNA部分缩合时PAHB / DNA混合物没有细胞毒性。这些结果表明星形低聚阳离子表面活性剂是基因转染的潜在缩合剂。

更新日期：2017-07-04

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