Surface Desorption Dielectric-Barrier Discharge Ionization Mass Spectrometry,Analytical Chemistry

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Surface Desorption Dielectric-Barrier Discharge Ionization Mass Spectrometry
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-06-30 00:00:00 , DOI: 10.1021/acs.analchem.7b00323
Hong Zhang _{1,

2} , Jie Jiang ₃ , Na Li ₃ , Ming Li ₄ , Yingying Wang ₃ , Jing He ₃ , Hong You _{1,

2,

3}

Affiliation

A variant of dielectric-barrier discharge named surface desorption dielectric-barrier discharge ionization (SDDBDI) mass spectrometry was developed for high-efficiency ion transmission and high spatial resolution imaging. In SDDBDI, a tungsten nanotip and the inlet of the mass spectrometer are used as electrodes, and a piece of coverslip is used as a sample plate as well as an insulating dielectric barrier, which simplifies the configuration of instrument and thus the operation. Different from volume dielectric-barrier discharge (VDBD), the microdischarges are generated on the surface at SDDBDI, and therefore the plasma density is extremely high. Analyte ions are guided directly into the MS inlet without any deflection. This configuration significantly improves the ion transmission efficiency and thus the sensitivity. The dependence of sensitivity and spatial resolution of the SDDBDI on the operation parameters were systematically investigated. The application of SDDBDI was successfully demonstrated by analysis of multiple species including amino acids, pharmaceuticals, putative cancer biomarkers, and mixtures of both fatty acids and hormones. Limits of detection (S/N = 3) were determined to be 0.84 and 0.18 pmol, respectively, for the analysis of l-alanine and metronidazole. A spatial resolution of 22 μm was obtained for the analysis of an imprinted cyclophosphamide pattern, and imaging of a “T” character was successfully demonstrated under ambient conditions. These results indicate that SDDBDI has high-efficiency ion transmission, high sensitivity, and high spatial resolution, which render it a potential tool for mass spectrometry imaging.

中文翻译：

表面解吸电介质阻挡放电电离质谱

研制了一种名为表面解吸介电屏障电离（SDDBDI）质谱的介电屏障放电变体，用于高效离子传输和高空间分辨率成像。在SDDBDI中，钨纳米尖端和质谱仪的入口用作电极，一片盖玻片用作样品板以及绝缘介电屏障，从而简化了仪器的配置，从而简化了操作。与体积介质阻挡放电（VDBD）不同，SDDBDI会在表面上产生微放电，因此等离子体密度非常高。分析物被直接导入MS进样口，没有任何偏转。这种配置显着提高了离子传输效率，从而提高了灵敏度。系统地研究了SDDBDI的灵敏度和空间分辨率对操作参数的依赖性。通过分析多种物种，包括氨基酸，药物，推定的癌症生物标志物以及脂肪酸和激素的混合物，成功证明了SDDBDI的应用。检测限（S / N = 3）分别用于分析1-丙氨酸和甲硝唑，分别为0.84和0.18 pmol 。获得22μm的空间分辨率，用于分析印迹的环磷酰胺图案，并在环境条件下成功展示了“ T”字的成像。这些结果表明SDDBDI具有高效率的离子传输，高灵敏度和高空间分辨率，这使其成为质谱成像的潜在工具。

更新日期：2017-07-02

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