3,5-Bis-(trifluoromethyl)phenyl isothiocyanate, was used as a convenient reagent for the derivatization of histamine, tyramine, tryptamine and 2-phenylethylamine, which eliminates the purification step. The obtained derivatives were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Additionally, the total amount of these amines was determined by ¹⁹F nuclear magnetic resonance analysis. The procedures were optimized and validated for linearity, limit of detection and quantification, intra- and inter-day precision and recovery resulting in good reproducibility and accuracy. The reagent was applied for determination of the above mentioned biogenic amines in beverages, and permitted an undemanding separation and determination of the derivatives. Moreover, it is a convenient reagent for analysis of the total amine content by quantitative ¹⁹F NMR.

中文翻译：

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3,5-双-（三氟甲基）苯基异硫氰酸酯在LC串联质谱和19F NMR测定选定的生物胺中的应用

3,5-双-（三氟甲基）苯基异硫氰酸酯用作组胺，酪胺，色胺和2-苯基乙胺衍生化的方便试剂，省去了纯化步骤。通过液相色谱-串联质谱法（LC-MS / MS）测定获得的衍生物。此外，这些胺的总量由¹⁹确定F核磁共振分析。对程序进行了优化和验证，以确保线性，检测和定量限，日内和日间精度以及回收率，从而实现了良好的可重复性和准确性。该试剂用于测定饮料中的上述生物胺，并可以不费力地分离和测定衍生物。此外，它是用于通过定量¹⁹ F NMR分析总胺含量的方便试剂。