Cpf1 is a CRISPR effector protein that has greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a single RNA polymerase II–driven RNA transcript expressed in mammalian cells. This property simplifies modification of multiple genomic targets and can be used to increase the efficiency of Cpf1-mediated editing.

中文翻译：

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Cpf1蛋白从哺乳动物细胞中的mRNA转录物中切除CRISPR RNA

Cpf1是一种CRISPR效应蛋白，在基因组编辑应用中比化脓链球菌Cas9（SpCas9）具有更高的特异性。在这里，我们显示Lachnospiraceae细菌（Lb）和Acidaminococus sp。（As）Cpf1直向同源物具有RNase活性，可以从哺乳动物细胞中表达的单个RNA聚合酶II驱动的RNA转录物中切除多个CRISPR RNA（crRNA）。此属性简化了多个基因组靶标的修饰，可用于提高Cpf1介导的编辑效率。