A surface‐enhanced Raman scattering (SERS) technique shows extraordinary features for a range of biological and biomedical applications. Herein, a series of novel bioorthogonal SERS nanoprobes were constructed with Gold nanoflower (AuNF) and Raman reporters, the signals of which were located in a Raman‐silent region of biological samples. AS1411 aptamer was also co‐conjugated with AuNF through a self‐assembled monolayer coverage strategy. Multiplex SERS imaging using these nanoprobes with three different bioorthogonal small‐molecule Raman reporters is successfully achieved with high multiplexing capacity in a biologically Raman‐silent region. These Raman nanoprobes co‐conjugated with AS1411 showed high affinity for tumor cells with overexpressed nucleolin and can be used for selective tumor cell screening and tissue imaging.

中文翻译：

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用于正交光谱检测，肿瘤细胞靶向和组织成像的生物正交SERS纳米探针

表面增强拉曼散射（SERS）技术在一系列生物和生物医学应用中显示出非凡的功能。本文中，用金纳米花（AuNF）和拉曼报道分子构建了一系列新型的生物正交SERS纳米探针，其信号位于生物样品的拉曼沉默区域。AS1411适体还通过自组装单层覆盖策略与AuNF共轭。使用这些纳米探针与三种不同的生物正交小分子拉曼报道分子进行多重SERS成像，可以在生物拉曼沉默区域实现高复用能力。这些与AS1411共轭的拉曼纳米探针对核仁过表达的肿瘤细胞显示出高度亲和力，可用于选择性肿瘤细胞筛查和组织成像。