基于液基的免疫传感器在临床标本中诊断肺结核,ACS Applied Materials & Interfaces

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基于液基的免疫传感器在临床标本中诊断肺结核
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2017-06-15 00:00:00 , DOI: 10.1021/acsami.7b06189
Hyeong Jin Kim ₁ , Jinseob Rim ₁ , Chang-Hyun Jang ₁

Affiliation

结核病虽然很容易预防和治愈，但却是一个严重的全球性健康问题。结核病的流行是由于缺乏简单，快速，便宜和有效的结核病诊断检测所致。在这项研究中，我们建立了一种新型的基于液晶（LC）的免疫传感器来诊断临床标本中的TB。将临床血清样品在固定有TB抗原的底物上孵育，并使用偏光显微镜观察其光学LC反应。抗结核抗体与固定有TB抗原的表面的特异性结合仅发生在来自TB患者的临床标本中，从而导致LC方向的破坏。随后是LC光学外观从均匀到随机的显着变化。然而，孵育来自健康人或潜在结核病患者的临床血清样本时，LC的方向保持一致。通过改变光学LC图像，在76％的TB患者中，本文正确地鉴定出患者的血清中具有针对TB的抗体。正确鉴定出91％没有结核病的健康人没有结核病抗体。因此，这种基于LC的免疫传感器是一个很有前途的平台，特别是在临床TB诊断中，它不需要复杂的临床标本制备或复杂的仪器。

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Liquid-Crystal-Based Immunosensor for Diagnosis of Tuberculosis in Clinical Specimens

Tuberculosis (TB) is a serious global health problem, although it is easily preventable and curable. The prevalence of TB is caused by a lack of simple, rapid, cheap, and effective TB diagnostic tests. In this study, we have established a novel liquid crystal (LC)-based immunosensor to diagnose TB in clinical specimens. The clinical serum samples were incubated on a TB antigen-immobilized substrate, and their optical LC responses were observed using a polarized light microscope. Specific binding of anti-TB antibodies to the TB antigen-immobilized surface only occurred in clinical specimens from TB patients, inducing the disruption of the orientation of LCs. This was followed by the distinctive change in the optical appearance of LCs from uniform to random. However, when clinical serum samples from healthy people or latent TB patients were incubated, the orientation of LCs remained uniform. Through the change of optical LC images, in 76% of TB patients, this essay correctly identified the patients as having antibodies to TB in their serums. 91% of healthy people free of TB were correctly identified as not having antibodies to TB. Thus, this LC-based immunosensor is a promising platform, particularly in clinical TB diagnostics, which does not require complicated preparation of clinical specimens or complex instrumentation.

更新日期：2017-06-15

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