In this study, nano-coppers were incorporated into an enzyme carrier, polyacrylonitrile/polyvinylidene fluoride (PAN/PVdF) electrospun fibrous membranes (EFMs). Laccase immobilized on PAN/PVdF/Cu EFMs prepared was used for the removal of 2,4,6-trichlorophenol from water. The physical and electrochemical properties of PAN/PVdF/Cu EFMs, as well as the efficiency of laccase immobilization and 2,4,6-TCP removal were investigated in detail. Scanning electron microscopy and transmission electron microscopy images show that the diameters of PAN/PVdF/Cu EFMs ranged from 200 nm to 500 nm. The immobilized laccase had a broader application pH and temperature range compared with free laccase. Laccase immobilized on PAN/PVdF/Cu EFMs showed significantly better performance on 2,4,6-TCP removal (95.4%) than that without nano-coppers (82.2%) (p < 0.05). It was because the incorporation of nano-coppers into the fibers facilitated the electron transfer during the process of catalytic reaction with laccase. In addition, it was demonstrated the lac-PAN/PVdF/Cu EFMs prepared in this work possessed high reusability. Therefore, the lac-PAN/PVdF/Cu EFMs have promising potential in industrial application for the removal of pollutants from water.

在这项研究中，将纳米铜结合到酶载体聚丙烯腈/聚偏二氟乙烯（PAN / PVdF）电纺纤维膜（EFMs）中。固定在制备的PAN / PVdF / Cu EFM上的漆酶用于从水中去除2,4,6-三氯苯酚。详细研究了PAN / PVdF / Cu EFM的物理和电化学性质，以及漆酶固定和去除2,4,6-TCP的效率。扫描电子显微镜和透射电子显微镜图像显示，PAN / PVdF / Cu EFM的直径范围为200 nm至500 nm。与游离漆酶相比，固定化漆酶具有更广泛的应用pH和温度范围。固定在PAN / PVdF / Cu EFM上的漆酶在去除2,4,6-TCP方面表现出显着更好的性能（95.4％），比没有纳米铜的表现出更好的性能（82.2％）（p <0.05）。这是因为在与漆酶的催化反应过程中，将纳米铜结合到纤维中促进了电子转移。此外，证明了在这项工作中制备的lac-PAN / PVdF / Cu EFM具有很高的可重复使用性。因此，lac-PAN / PVdF / Cu EFM在工业应用中具有从水中去除污染物的潜力。