Metabolic Effect of 1-Deoxynojirimycin from Mulberry Leaves on db/db Diabetic Mice Using Liquid Chromatography–Mass Spectrometry Based Metabolomics,Journal of Agricultural and Food Chemistry

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Metabolic Effect of 1-Deoxynojirimycin from Mulberry Leaves on db/db Diabetic Mice Using Liquid Chromatography–Mass Spectrometry Based Metabolomics
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2017-06-02 00:00:00 , DOI: 10.1021/acs.jafc.7b01766
Xue-Qin Hu , Kiran Thakur , Gui-Hai Chen ₁ , Fei Hu , Jian-Guo Zhang , Hong-Bin Zhang , Zhao-Jun Wei

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Metabolomics was applied to the liquid chromatography–mass spectrometry urinary metabolic profile of type 2 diabetes (T2DM) mice treated with mulberry 1-deoxynojirimycin (DNJ). The serum biochemical indicators related to T2DM like blood glucose, insulin, triglyceride, total cholesterol, nitrogen, malondialdehyde, and creatinine decreased significantly in the treated group. The histopathological changes in liver cells were marked by deformations and disruptions in central area of nuclei in DM mice, whereas DNJ treatment recovered regular liver cells with normal nuclei. Most of the metabolites of T2DM were significantly different from healthy controls in the bulk data generated. The level of 16 metabolites showed that the diabetic group was closer to the healthy group as the DNJ treatment time prolonged. Moreover, DNJ inhibited the activity of glucosidase on glucose, lipid, and amino acid metabolism. Our results showed the mechanism of DNJ treatment of T2DM and could fetch deep insights into the potent metabolite markers of the applied antidiabetic interventions.

中文翻译：

液相色谱-质谱联用研究桑叶中1-脱氧野rim霉素对db / db糖尿病小鼠的代谢作用

代谢组学被应用于用桑树1-脱氧野oji霉素（DNJ）治疗的2型糖尿病（T2DM）小鼠的液相色谱-质谱法尿代谢谱。与T2DM有关的血清生化指标如血糖，胰岛素，甘油三酸酯，总胆固醇，氮，丙二醛和肌酐在治疗组显着下降。DM小鼠肝细胞的组织病理学变化以核中心区域的变形和破坏为特征，而DNJ治疗则恢复了具有正常核的正常肝细胞。在生成的大量数据中，大多数T2DM代谢产物与健康对照显着不同。16种代谢物的水平表明，随着DNJ治疗时间的延长，糖尿病组更接近健康组。而且，DNJ抑制了葡萄糖苷酶对葡萄糖，脂质和氨基酸代谢的活性。我们的结果显示了DNJ治疗T2DM的机制，并且可以深入了解所应用的抗糖尿病干预措施的有效代谢物标记物。

更新日期：2017-06-02

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