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Sensitive Flow-through Immunoassay for Rapid Multiplex Determination of Cereal-borne Mycotoxins in Feed and Feed Ingredients
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2017-01-12 00:00:00 , DOI: 10.1021/acs.jafc.6b03172 Natalia V. Beloglazova 1 , Kinga Graniczkowska 1 , Emmanuel Njumbe Ediage 1 , Olga Averkieva 2 , Sarah De Saeger 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2017-01-12 00:00:00 , DOI: 10.1021/acs.jafc.6b03172 Natalia V. Beloglazova 1 , Kinga Graniczkowska 1 , Emmanuel Njumbe Ediage 1 , Olga Averkieva 2 , Sarah De Saeger 1
Affiliation
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An easy-to-operate membrane-based flow-through test for multiplex screening of four mycotoxins (zearalenone, deoxynivalenol, aflatoxin B1, and ochratoxin A) in a variety of cereal-based feed ingredients and compound feeds, such as wheat, barley, soybean, wheat bran, rice, rice bran, maize, rapeseed meal, and sunflower meal, and various types of complete feed (duckling feed, swine feed, broiler feed, piglet feed) was developed and validated. First, the antibodies were evaluated by enzyme-linked immunosorbent assay and then employed in the membrane rapid test. The cutoff levels for zearalenone, deoxynivalenol, aflatoxin B1, and ochratoxin A were 50, 200, 1, and 10 μg/kg, respectively, based on European regulations and consumers’ requirements. As sample pretreatment, consecutive steps of extraction, dilution, solid-phase extraction by addition of C18 sorbent, and final filtration of supernatant were followed. Both the sample preparation and the analysis procedure were simple, cost-effective, and easy to perform on-site in a nonlaboratory environment. The impact of sample processing on the result of the experiment was investigated supported by experimental design. The validation procedure was performed on the basis of Commission Regulation 2006/401/EC. The numbers of false-positive and false-negative outcomes were <5%, going along with the Commission Decision 2002/657/EC. Liquid chromatography–tandem mass spectrometry was performed as a confirmatory technique.
中文翻译：
灵敏的流通免疫分析法可快速多重测定饲料和饲料成分中的谷物传播的霉菌毒素
一种易于操作的基于膜的流穿测试，可对多种谷物类饲料原料和复合饲料（例如小麦，大麦）中的四种霉菌毒素（玉米赤霉烯酮，脱氧雪腐烯醇，黄曲霉毒素B 1和曲霉毒素A）进行多重筛选开发并验证了大豆，麦麸，大米，米糠，玉米，油菜籽粉和向日葵粉，以及各种类型的完整饲料（小鸭饲料，猪饲料，肉鸡饲料，仔猪饲料）。首先，通过酶联免疫吸附测定法评估抗体，然后将其用于膜快速测试中。玉米赤霉烯酮，脱氧雪腐烯醇，黄曲霉毒素B 1的临界水平根据欧洲法规和消费者要求，曲霉毒素A和曲霉毒素A分别为50、200、1和10μg/ kg。作为样品的预处理，依次进行提取，稀释，通过添加C18吸附剂进行固相提取以及最终过滤上清液的连续步骤。样品制备和分析程序都很简单，具有成本效益，并且易于在非实验室环境中现场进行。实验设计支持了样品处理对实验结果的影响。验证程序是根据委员会法规2006/401 / EC执行的。与委员会决定2002/657 / EC一致，假阳性和假阴性结果的数量小于5％。液相色谱-串联质谱法是一种验证性技术。
更新日期：2017-01-12
中文翻译：
灵敏的流通免疫分析法可快速多重测定饲料和饲料成分中的谷物传播的霉菌毒素
一种易于操作的基于膜的流穿测试，可对多种谷物类饲料原料和复合饲料（例如小麦，大麦）中的四种霉菌毒素（玉米赤霉烯酮，脱氧雪腐烯醇，黄曲霉毒素B 1和曲霉毒素A）进行多重筛选开发并验证了大豆，麦麸，大米，米糠，玉米，油菜籽粉和向日葵粉，以及各种类型的完整饲料（小鸭饲料，猪饲料，肉鸡饲料，仔猪饲料）。首先，通过酶联免疫吸附测定法评估抗体，然后将其用于膜快速测试中。玉米赤霉烯酮，脱氧雪腐烯醇，黄曲霉毒素B 1的临界水平根据欧洲法规和消费者要求，曲霉毒素A和曲霉毒素A分别为50、200、1和10μg/ kg。作为样品的预处理，依次进行提取，稀释，通过添加C18吸附剂进行固相提取以及最终过滤上清液的连续步骤。样品制备和分析程序都很简单，具有成本效益，并且易于在非实验室环境中现场进行。实验设计支持了样品处理对实验结果的影响。验证程序是根据委员会法规2006/401 / EC执行的。与委员会决定2002/657 / EC一致，假阳性和假阴性结果的数量小于5％。液相色谱-串联质谱法是一种验证性技术。
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