动态DNA折纸装置，用于测量压缩力,ACS Nano

当前位置： X-MOL 学术 › ACS Nano › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

动态DNA折纸装置，用于测量压缩力
ACS Nano ( IF 15.8 ) Pub Date : 2017-06-05 00:00:00 , DOI: 10.1021/acsnano.6b07097
Michael W. Hudoba ₁ , Yi Luo , Angelita Zacharias , Michael G. Poirier , Carlos E. Castro

Affiliation

具有可感测和响应局部环境的程序化结构动力学的自组装纳米设备的能力，可以在分子机器人，纳米制造和纳米医学等领域实现变革性的应用。生物分子的响应功能通常是由与局部环境高度敏感的相互作用介导的构象分布的变化驱动的。在这里，我们通过将固有的纳米级结构动力学（纳米动力学）工程化为DNA器件来模仿这种方法，该DNA器件显示出构象分布，其中包括两个稳定态，该两个稳定态被过渡态分隔开，其中能垒高度在热能k _{B的范围内}Ť= 4.1 pN·nm，实现状态之间的自发跃迁。我们进一步建立设计原理，通过取代一些DNA链组件来调节平衡和动力学行为。我们使用单分子Förster共振能量转移测量结果来显示，在存在分子拥挤剂的情况下，这些纳米动力学性质对亚微微网民耗尽力敏感，并且该装置可以测量分辨率约为100 fN的耗尽力。我们预计，这种工程化的纳米动力学DNA装置的方法将使分子规模的系统能够以极高的灵敏度感应并响应其局部环境。

"点击查看英文标题和摘要"

Dynamic DNA Origami Device for Measuring Compressive Depletion Forces

The ability to self-assemble nanodevices with programmed structural dynamics that can sense and respond to the local environment could enable transformative applications in fields including molecular robotics, nanomanufacturing, and nanomedicine. The responsive function of biomolecules is often driven by alterations in conformational distributions mediated by highly sensitive interactions with the local environment. Here, we mimic this approach by engineering inherent nanoscale structural dynamics (nanodynamics) into a DNA device that exhibits a distribution of conformations including two stable states separated by a transition state where the energy barrier height is on the scale of the thermal energy, k_BT = 4.1 pN·nm, enabling spontaneous transitions between states. We further establish design principles to regulate the equilibrium and kinetic behavior by substituting a few DNA strand components. We use single-molecule Förster resonance energy transfer measurements to show these nanodynamic properties are sensitive to sub-piconewton depletion forces in the presence of molecular crowding agents, and the device can measure depletion forces with a resolution of ∼100 fN. We anticipate that this approach of engineering nanodynamic DNA devices will enable molecular-scale systems that sense and respond to their local environment with extremely high sensitivity.

更新日期：2017-06-28

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南