Enediol-Ligands-Encapsulated Liposomes Enables Sensitive Immunoassay: A Proof-of-Concept for General Liposomes-Based Photoelectrochemical Bioanalysis,Analytical Chemistry

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Enediol-Ligands-Encapsulated Liposomes Enables Sensitive Immunoassay: A Proof-of-Concept for General Liposomes-Based Photoelectrochemical Bioanalysis
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-06-08 00:00:00 , DOI: 10.1021/acs.analchem.7b01291
Li-Ping Mei ₁ , Fei Liu ₁ , Jian-Bin Pan ₁ , Wei-Wei Zhao _{1,

2} , Jing-Juan Xu ₁ , Hong-Yuan Chen ₁

Affiliation

Sensitive photoelectrochemical (PEC) bioanalysis usually relies on enzyme-assisted signal amplification. This work describes the first proof-of-concept study for liposome-based PEC bioanalysis. Specifically, unilamellar liposomes were prepared and then utilized to carry the enediol-ligands and antibodies within their internal cavities and upon their external surfaces, respectively. On the other hand, the 96-well plate was used for accommodating the sandwich immunocomplexing, and then the confined liposomes were directed to release the encapsulated enediol-ligands into an individual well. The subsequent in situ sensitization of the TiO₂ nanoparticles (NPs) electrode was then used to transduce the recognition events. This facile strategy allows for sensitive immunoassay without the involvement of laborious electrode fabrication and enzymatic amplification. Importantly, the protocol can be extended as a general PEC method for numerous other targets of interest. We believe this work could offer a new perspective for the rational implementation of various liposome complexes for novel PEC bioanalysis.

中文翻译：

乙二醇配体封装的脂质体实现灵敏的免疫测定：基于脂质体的基于光电化学生物分析的概念验证

灵敏的光电化学（PEC）生物分析通常依赖于酶辅助信号放大。这项工作描述了基于脂质体的PEC生物分析的第一个概念验证研究。具体而言，制备单层脂质体，然后将其用于在其内腔中和在其外表面上分别携带烯二醇-配体和抗体。另一方面，将96孔板用于容纳夹心免疫复合物，然后将限制的脂质体引导以将包封的烯二醇配体释放到单个孔中。TiO ₂的后续原位敏化然后使用纳米颗粒（NPs）电极转导识别事件。这种简便的策略无需进行繁琐的电极制备和酶促扩增即可进行灵敏的免疫测定。重要的是，该协议可以作为通用的PEC方法扩展为许多其他感兴趣的目标。我们相信这项工作可以为新型脂质体生物分析中各种脂质体复合物的合理实施提供新的视角。

更新日期：2017-06-10

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