Toward artifact-free data in Hadamard transform-based double multiplexing of ion mobility-Orbitrap mass spectrometry,Analyst

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Toward artifact-free data in Hadamard transform-based double multiplexing of ion mobility-Orbitrap mass spectrometry
Analyst ( IF 3.6 ) Pub Date : 2017-04-10 00:00:00 , DOI: 10.1039/c7an00289k
Meghasyam Tummalacherla _{1,

2,

3,

4} , Sandilya V. B. Garimella _{1,

2,

3,

4} , Spencer A. Prost _{1,

2,

3,

4} , Yehia M. Ibrahim _{1,

2,

3,

4}

Affiliation

The integration of ion mobility spectrometry (IMS) with a trap-based mass spectrometer (MS) such as Orbitrap using the dual gate approach suffers from low duty cycle. Efforts to improve the duty cycle involve the utilization of Hadamard transform-based double multiplexing which significantly improves the signal-to-noise ratio and duty cycle of the ion mobility-Orbitrap mass spectrometry (IM-Orbitrap MS) platform. However, artifacts and noise in the demultiplexed data significantly reduce the data quality and negate the benefits of multiplexing. We propose a new approach that identifies the true IM peaks and helps in eliminating the artifacts in the demultiplexed data leading to a decrease in false identifications in subsequent data processing. After the application of the algorithm, the quality of the IM-Orbitrap MS measurements was greatly improved because of the reduction in artifacts.

中文翻译：

在基于Hadamard变换的离子迁移率-Orbitrap质谱双重复用中获得无伪影的数据

使用双门方法将离子迁移谱（IMS）与基于陷阱的质谱仪（MS）（例如Orbitrap）集成在一起时，占空比很低。改善占空比的工作包括利用基于Hadamard变换的双重复用技术，该技术显着提高了离子迁移率Orbitrap质谱仪（IM-Orbitrap MS）平台的信噪比和占空比。但是，解复用后的数据中的伪影和噪声会大大降低数据质量，并抵消了复用的好处。我们提出了一种新的方法，该方法可以识别真实的IM峰值，并有助于消除多路分解数据中的伪像，从而减少后续数据处理中的错误识别。应用该算法后，

更新日期：2017-05-31

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