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Effects of Biomass Accessibility and Klason Lignin Contents during Consolidated Bioprocessing in Populus trichocarpa
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2017-04-26 00:00:00 , DOI: 10.1021/acssuschemeng.7b00449 Hannah Akinosho 1, 2 , Alexandru Dumitrache 1, 3 , Jace Natzke 1, 3 , Wellington Muchero 4 , Sara S. Jawdy 4 , Gerald A. Tuskan 4 , Steven D. Brown 1, 3 , Arthur J. Ragauskas 1, 3, 5
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2017-04-26 00:00:00 , DOI: 10.1021/acssuschemeng.7b00449 Hannah Akinosho 1, 2 , Alexandru Dumitrache 1, 3 , Jace Natzke 1, 3 , Wellington Muchero 4 , Sara S. Jawdy 4 , Gerald A. Tuskan 4 , Steven D. Brown 1, 3 , Arthur J. Ragauskas 1, 3, 5
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The bacterium Clostridium thermocellum offers a distinct and integrated approach to ethanol production through consolidated bioprocessing (CBP). The Simons’ stain technique, which assays the accessibility of lignocellulosic biomass, has been traditionally applied to fungal cellulase systems; however, its application to CBP has not been fully explored. For this reason, the structural properties of eight Populus trichocarpa with either high or low biomass densities were compared to determine bioconversion differences during separate hydrolysis and fermentation (SHF) and CBP with C. thermocellum. Simons’ staining generally identifies low density poplar as more accessible than high density poplar. Additionally, low density P. trichocarpa generally contained less Klason lignin than high density poplar. SHF and CBP treatments consistently identified BESC-7 (high density, low accessibility, low surface roughness) as a low ethanol yielding biomass and GW-9914 (low density, high accessibility, high surface roughness) as a high ethanol yielding biomass. Upon further investigation, BESC-7 also contained a high Klason lignin content (∼25%), while GW-9914 had a low lignin content (∼20%). Cellulose degree of polymerization (DP) measurements exhibited a weak linear correlation with accessibility (r2 = 0.17). Therefore, the ethanol yields were correlated with accessibility and lignin content extremes but not cellulose DP.
中文翻译：
山毛杨综合生物加工过程中生物量可及性和克拉森木质素含量的影响
细菌热纤梭菌提供通过联合生物加工（CBP）的独特和综合方法生产乙醇。西蒙斯的染色技术用于检测木质纤维素生物质的可及性，传统上已应用于真菌纤维素酶系统。然而，其在CBP中的应用尚未得到充分探索。由于这个原因，比较了八个具有高或低生物量密度的毛果杨的结构特性，以确定在单独的水解和发酵（SHF）和含热纤维梭菌的CBP期间的生物转化差异。西蒙斯（Simons）的染色通常将低密度杨树比高密度杨树更容易接近。此外，低密度毛果杨通常所含的Klason木质素少于高密度的杨木。SHF和CBP处理一致地将BESC-7（高密度，低可及性，低表面粗糙度）确定为低乙醇产量的生物质，并将GW-9914（低密度，高可及性，高表面粗糙度）确定为高乙醇产量的生物质。经进一步调查，BESC-7的Klason木质素含量也很高（约25％），而GW-9914的木质素含量却很低（约20％）。纤维素聚合度（DP）的测量显示出与可及性之间的弱线性相关性（r 2 = 0.17）。因此，乙醇产量与可及性和木质素含量的极端值相关，而与纤维素DP无关。
更新日期：2017-05-31
中文翻译：
山毛杨综合生物加工过程中生物量可及性和克拉森木质素含量的影响
细菌热纤梭菌提供通过联合生物加工（CBP）的独特和综合方法生产乙醇。西蒙斯的染色技术用于检测木质纤维素生物质的可及性，传统上已应用于真菌纤维素酶系统。然而，其在CBP中的应用尚未得到充分探索。由于这个原因，比较了八个具有高或低生物量密度的毛果杨的结构特性，以确定在单独的水解和发酵（SHF）和含热纤维梭菌的CBP期间的生物转化差异。西蒙斯（Simons）的染色通常将低密度杨树比高密度杨树更容易接近。此外，低密度毛果杨通常所含的Klason木质素少于高密度的杨木。SHF和CBP处理一致地将BESC-7（高密度，低可及性，低表面粗糙度）确定为低乙醇产量的生物质，并将GW-9914（低密度，高可及性，高表面粗糙度）确定为高乙醇产量的生物质。经进一步调查，BESC-7的Klason木质素含量也很高（约25％），而GW-9914的木质素含量却很低（约20％）。纤维素聚合度（DP）的测量显示出与可及性之间的弱线性相关性（r 2 = 0.17）。因此，乙醇产量与可及性和木质素含量的极端值相关，而与纤维素DP无关。
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