Endo-α-Mannosidase-Catalyzed Transglycosylation,ChemBioChem

当前位置： X-MOL 学术 › ChemBioChem › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Endo-α-Mannosidase-Catalyzed Transglycosylation
ChemBioChem ( IF 2.6 ) Pub Date : 2017-05-29 11:05:37 , DOI: 10.1002/cbic.201700111
Shogo Iwamoto ₁ , Yuta Kasahara ₁ , Yayoi Yoshimura ₁ , Akira Seko ₂ , Yoichi Takeda ₃ , Yukishige Ito _{2,

4} , Kiichiro Totani ₅ , Ichiro Matsuo ₁

Affiliation

In order for facilitating the synthesis of oligosaccharides, transglycosylation reactions mediated by glycoside hydrolases have been studied in various contexts. In this study, we examined the transglycosylating activity of a Golgi endo-α-mannosidase. We prepared various glycosyl donors and acceptors, and recombinant human Golgi endo-α-mannosidase and its various mutants were expressed. The enzyme was able to mediate transglycosylation from α-glycosyl-fluorides. Systematic screening of various point mutants revealed that the E407D mutant had excellent transglycosylation activity and extremely low hydrolytic activity. Substrate specificity analysis revealed that minimum motif required for glycosyl acceptor is Manα1– 2Man. The synthetic utility of the enzyme was demonstrated by generation of a high-mannose-type undecasaccharide (Glc₁Man₉GlcNAc₂).

中文翻译：

内切α-甘露糖苷酶催化的转糖基化

为了促进寡糖的合成，已经在各种情况下研究了由糖苷水解酶介导的转糖基化反应。在这项研究中，我们研究了高尔基的transglycosylating活动内- α甘露糖苷酶。我们准备了各种糖供体和受体和重组人高尔基内表达了-α-甘露糖苷酶及其各种突变体。该酶能够介导来自α-糖基氟化物的转糖基化。对各种点突变体的系统筛选显示，E407D突变体具有出色的转糖基化活性和极低的水解活性。底物特异性分析表明，糖基受体所需的最小基序为Manα1-2Man。通过产生高甘露糖型十一碳糖（Glc ₁ Man ₉ GlcNAc ₂）证明了该酶的合成效用。

更新日期：2017-05-30

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南