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Gold Nanoparticle Based Hairpin-Locked-DNAzyme Probe for Amplified miRNA Imaging in Living Cells
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-05-15 00:00:00 , DOI: 10.1021/acs.analchem.7b00174 Yanjing Yang 1 , Jin Huang 1 , Xiaohai Yang 1 , Xiaoxiao He 1 , Ke Quan 1 , Nuli Xie 1 , Min Ou 1 , Kemin Wang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-05-15 00:00:00 , DOI: 10.1021/acs.analchem.7b00174 Yanjing Yang 1 , Jin Huang 1 , Xiaohai Yang 1 , Xiaoxiao He 1 , Ke Quan 1 , Nuli Xie 1 , Min Ou 1 , Kemin Wang 1
Affiliation
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A new class of intracellular nanoprobe, termed AuNP-based hairpin-locked-DNAzyme probe, was developed to sense miRNA in living cells. Briefly, it consists of an AuNP and hairpin-locked-DNAzyme strands. In the absence of target miRNA, the hairpin-locked-DNAzyme strand forms a hairpin structure by intramolecular hybridization, which could inhibit the catalytic activity of DNAzyme strand and the fluorescence is quenched by the AuNP. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active secondary structure in the catalytic cores to yield an “active” DNAzyme, which then cleaves the self-strand with the assist of Mg2+. The cleaved two shorter DNA fragments are separated with the target. As a result, the fluorophores are released from the AuNP and the fluorescence is enhanced. Meanwhile, the target is also released and binds to another hairpin-locked-DNAzyme strand to drive another cycle of activation. In such a way, the target-recycling amplification leads to significant signal enhancement and thus offers high detection sensitivity.
中文翻译：
基于金纳米粒子的发夹锁定DNAzyme探针，用于在活细胞中扩增miRNA成像
开发了一种新型的细胞内纳米探针，称为基于AuNP的发夹锁定DNAzyme探针，用于检测活细胞中的miRNA。简而言之，它由AuNP和发夹固定的DNAzyme链组成。在不存在靶标miRNA的情况下，发夹固定的DNAzyme链通过分子内杂交形成发夹结构，这可能抑制DNAzyme链的催化活性，并且荧光被AuNP淬灭。但是，在存在靶标的情况下，靶标探针杂交可以打开发夹并在催化核心中形成活性二级结构，从而产生“活性” DNA酶，然后在Mg 2+的帮助下裂解自链。。切割的两个较短的DNA片段与靶标分开。结果，荧光团从AuNP释放并且荧光增强。同时，靶标也被释放并与另一条发夹锁定的DNAzyme链结合，以驱动另一个激活周期。以这种方式，靶物循环的扩增导致显着的信号增强，并因此提供了高的检测灵敏度。
更新日期：2017-05-24
中文翻译：
基于金纳米粒子的发夹锁定DNAzyme探针，用于在活细胞中扩增miRNA成像
开发了一种新型的细胞内纳米探针，称为基于AuNP的发夹锁定DNAzyme探针，用于检测活细胞中的miRNA。简而言之，它由AuNP和发夹固定的DNAzyme链组成。在不存在靶标miRNA的情况下，发夹固定的DNAzyme链通过分子内杂交形成发夹结构，这可能抑制DNAzyme链的催化活性，并且荧光被AuNP淬灭。但是，在存在靶标的情况下，靶标探针杂交可以打开发夹并在催化核心中形成活性二级结构，从而产生“活性” DNA酶，然后在Mg 2+的帮助下裂解自链。。切割的两个较短的DNA片段与靶标分开。结果，荧光团从AuNP释放并且荧光增强。同时，靶标也被释放并与另一条发夹锁定的DNAzyme链结合，以驱动另一个激活周期。以这种方式，靶物循环的扩增导致显着的信号增强，并因此提供了高的检测灵敏度。
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