载有全氟化碳的超孔介孔结构二氧化硅纳米颗粒的氧传感,ACS Nano

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载有全氟化碳的超孔介孔结构二氧化硅纳米颗粒的氧传感
ACS Nano ( IF 15.8 ) Pub Date : 2017-05-15 00:00:00 , DOI: 10.1021/acsnano.7b01006
Amani L. Lee ₁ , Clifford T. Gee ₁ , Bradley P. Weegman ₁ , Samuel A. Einstein ₁ , Adam R. Juelfs ₁ , Hattie L. Ring ₁ , Katie R. Hurley ₁ , Sam M. Egger ₁ , Garrett Swindlehurst ₁ , Michael Garwood ₁ , William C. K. Pomerantz ₁ , Christy L. Haynes ₁

Affiliation

氧稳态在调节生物学功能中很重要。可以通过测量氧气水平来监测疾病的进展，从而为设计治疗方法提供信息。组织氧合的非侵入性测量需要开发具有最小的不良影响和方便地检测目标特征的工具。氟磁共振成像（¹⁹F MRI）利用全氟化碳（PFC）液体的固有特性进行解剖学成像，细胞跟踪和氧感测。然而，全氟碳化合物的高度疏水和疏脂性质需要形成用于生物学研究的乳剂，尽管稳定这些乳剂一直具有挑战性。为了增强全氟化碳的稳定性和生物负载，一种选择是将全氟化碳液体掺入生物相容的介孔二氧化硅纳米粒子的内部空间。在这里，我们开发了装载全氟化碳的超孔介孔结构二氧化硅纳米颗粒（PERFUMNs），作为¹⁹ F MRI可检测的氧传感探针。超孔介孔结构的二氧化硅纳米粒子（UMN）具有较大的内部空腔（平均= 1.8 cm ³ g ^–1），提高了PFC的平均装载效率17％，满足了影像学研究所需的阈值氟浓度。全氟15冠5醚PERFUMNs每个PFC分子的等效核最高，在16.4 T时，基于自旋-晶格（T ₁）弛豫的氧敏感性为0.0032 mmHg ^–1 s ^–1。合成UMN而不是传统的原位核壳合成方法，可以使用一种材料制造多种PFC液体。UMNs的生物相容性和可调谐化学性质与PFC的固有特性相结合，使PERFUMNs成为MRI传感器，具有改进的稳定性，可用于解剖学成像，细胞跟踪和代谢光谱学。

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Oxygen Sensing with Perfluorocarbon-Loaded Ultraporous Mesostructured Silica Nanoparticles

Oxygen homeostasis is important in the regulation of biological function. Disease progression can be monitored by measuring oxygen levels, thus producing information for the design of therapeutic treatments. Noninvasive measurements of tissue oxygenation require the development of tools with minimal adverse effects and facile detection of features of interest. Fluorine magnetic resonance imaging (¹⁹F MRI) exploits the intrinsic properties of perfluorocarbon (PFC) liquids for anatomical imaging, cell tracking, and oxygen sensing. However, the highly hydrophobic and lipophobic properties of perfluorocarbons require the formation of emulsions for biological studies, though stabilizing these emulsions has been challenging. To enhance the stability and biological loading of perfluorocarbons, one option is to incorporate perfluorocarbon liquids into the internal space of biocompatible mesoporous silica nanoparticles. Here, we developed perfluorocarbon-loaded ultraporous mesostructured silica nanoparticles (PERFUMNs) as ¹⁹F MRI detectable oxygen-sensing probes. Ultraporous mesostructured silica nanoparticles (UMNs) have large internal cavities (average = 1.8 cm³ g^–1), facilitating an average 17% loading efficiency of PFCs, meeting the threshold fluorine concentrations needed for imaging studies. Perfluoro-15-crown-5-ether PERFUMNs have the highest equivalent nuclei per PFC molecule and a spin–lattice (T₁) relaxation-based oxygen sensitivity of 0.0032 mmHg^–1 s^–1 at 16.4 T. The option of loading PFCs after synthesizing UMNs, rather than traditional in situ core–shell syntheses, allows for use of a broad range of PFC liquids from a single material. The biocompatible and tunable chemistry of UMNs combined with the intrinsic properties of PFCs makes PERFUMNs a MRI sensor with potential for anatomical imaging, cell tracking, and metabolic spectroscopy with improved stability.

更新日期：2017-05-23

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