A Freeze‐Concentration and Polyampholyte‐Modified Liposome‐Based Antigen‐Delivery System for Effective Immunotherapy,Advanced Healthcare Materials

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A Freeze‐Concentration and Polyampholyte‐Modified Liposome‐Based Antigen‐Delivery System for Effective Immunotherapy
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2017-05-11 , DOI: 10.1002/adhm.201700207
Sana Ahmed ₁ , Satoshi Fujita ₂ , Kazuaki Matsumura ₁

Affiliation

Immunotherapy is an exciting new approach to cancer treatment. The development of a novel freeze‐concentration method is described that could be applicable in immunotherapy. The method involves freezing cells in the presence of pH‐sensitive, polyampholyte‐modified liposomes with encapsulated ovalbumin (OVA) as the antigen. In RAW 264.7 cells, compared to unfrozen, freeze‐concentration of polyampholyte‐modified liposomes encapsulating OVA resulted in efficient OVA uptake and also allowed its delivery to the cytosol. Efficient delivery of OVA to the cytosol was shown to be partly due to the pH‐dependence of the polyampholyte‐modified liposomes. Cytosolic OVA delivery also resulted in significant up‐regulation of the major histocompatibility complex class I pathway through cross‐stimulation, as well as an increase in the release of IL‐1β, IL‐6, and TNF‐α. The results demonstrate that the combination of a simple freeze‐concentration method and polyampholyte‐modified liposomes might be useful in future immunotherapy applications.

中文翻译：

冷冻浓缩和多两性修饰脂质体为基础的抗原递送系统，可用于有效的免疫治疗

免疫疗法是一种令人兴奋的癌症治疗新方法。描述了一种新颖的冷冻浓缩方法的开发，该方法可用于免疫治疗。该方法涉及在pH敏感的，经多两性修饰的脂质体包裹的卵清蛋白（OVA）作为抗原的情况下冷冻细胞。在RAW 264.7细胞中，与未冻结的相比，包裹OVA的经两性电解质修饰的脂质体的冷冻浓缩可有效吸收OVA，也可将其传递到细胞质中。OVA有效地传递到细胞质中的部分原因是由于多两性修饰脂质体的pH依赖性。通过交叉刺激，胞质OVA的递送还导致主要的组织相容性复合物I类通路显着上调，并且IL-1β，IL-6，和TNF-α。结果表明，简单的冷冻浓缩方法和多两性修饰脂质体的组合可能在未来的免疫治疗应用中有用。

更新日期：2017-05-11

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