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Ring-Oven Washing Technique Integrated Paper-based Immunodevice for Sensitive Detection of Cancer Biomarker
Analytical Chemistry ( IF 6.7 ) Pub Date : 2015-07-15 00:00:00 , DOI: 10.1021/acs.analchem.5b01814
Wei Liu 1 , Yumei Guo 1 , Mei Zhao 1 , Huifang Li 1 , Zhujun Zhang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2015-07-15 00:00:00 , DOI: 10.1021/acs.analchem.5b01814
Wei Liu 1 , Yumei Guo 1 , Mei Zhao 1 , Huifang Li 1 , Zhujun Zhang 1
Affiliation
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A paper-based microfluidic immunodevice has recently attracted considerable interest for point-of-care testing (POCT) and a washing procedure was used as a standard procedure in immunoassay to eliminate the nonspecific binding protein from a paper surface. However, the traditional washing method cannot get rid of the nonspecific binding protein more completely to get a lower background. In this work, a novel washing strategy with a ring-oven technique integrated on a paper-based immunodevice was presented, which can effectively wash a nonspecific binding protein and enable a low background for sensitive detection of the carcinoembryonic antigen (CEA). By immobilizing the antibody on the detection area and incorporating the temperature-controlled ring-oven under the paper-based device, the continuous washing solution can carry the nonspecific binding protein to the waste area freely by capillary force and then the waste area dried quickly by heating. The paper device, which is matched to the size of the ring-oven, is composed of eight microfluidic channels by the simple and rapid paper-cutting fabrication method. With the HRP-catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB)–H2O2 colorimetric detection method, a lower detection limit of 0.03 ng/mL CEA can be obtained by enzyme-linked immunosorbent assay (ELISA). The washing efficiency for the nonspecific binding protein was improved a lot compared to the traditional washing methods, and the established paper-based device can be used in the determination of CEA in human serum with high sensitivity. The paper-based device provides a new washing strategy for sensitive immunoassay and point-of-care diagnostics.
中文翻译：
环形烤箱清洗技术集成了基于纸的免疫装置，可灵敏地检测癌症生物标志物
纸基微流体免疫装置最近对即时检验（POCT）引起了极大的兴趣，并且将洗涤程序作为免疫测定中的标准程序，以消除纸表面的非特异性结合蛋白。然而，传统的洗涤方法不能更完全地去除非特异性结合蛋白以得到较低的背景。在这项工作中，提出了一种新的洗涤策略，该技术采用了集成在基于纸张的免疫装置上的环形烤箱技术，可以有效地洗涤非特异性结合蛋白并实现低背景的癌胚抗原（CEA）灵敏检测。通过将抗体固定在检测区域上，并将温度可控的环形烤箱并入纸基设备中，连续洗涤液可以通过毛细管力将非特异性结合蛋白自由携带到废物区，然后通过加热迅速干燥废物区。通过简单快速的剪纸制造方法，与环形烘箱尺寸匹配的纸装置由八个微流体通道组成。用HRP催化3,3'，5,5'-四甲基联苯胺（TMB）–H2 O 2比色检测法可通过酶联免疫吸附法（ELISA）获得0.03 ng / mL CEA的下限。与传统的洗涤方法相比，非特异性结合蛋白的洗涤效率有了很大提高，所建立的纸基设备可以高灵敏度地用于测定人血清中的CEA。基于纸张的设备为灵敏的免疫分析和即时诊断提供了一种新的洗涤策略。
更新日期：2015-07-15
中文翻译：
环形烤箱清洗技术集成了基于纸的免疫装置，可灵敏地检测癌症生物标志物
纸基微流体免疫装置最近对即时检验（POCT）引起了极大的兴趣，并且将洗涤程序作为免疫测定中的标准程序，以消除纸表面的非特异性结合蛋白。然而，传统的洗涤方法不能更完全地去除非特异性结合蛋白以得到较低的背景。在这项工作中，提出了一种新的洗涤策略，该技术采用了集成在基于纸张的免疫装置上的环形烤箱技术，可以有效地洗涤非特异性结合蛋白并实现低背景的癌胚抗原（CEA）灵敏检测。通过将抗体固定在检测区域上，并将温度可控的环形烤箱并入纸基设备中，连续洗涤液可以通过毛细管力将非特异性结合蛋白自由携带到废物区，然后通过加热迅速干燥废物区。通过简单快速的剪纸制造方法，与环形烘箱尺寸匹配的纸装置由八个微流体通道组成。用HRP催化3,3'，5,5'-四甲基联苯胺（TMB）–H2 O 2比色检测法可通过酶联免疫吸附法（ELISA）获得0.03 ng / mL CEA的下限。与传统的洗涤方法相比，非特异性结合蛋白的洗涤效率有了很大提高，所建立的纸基设备可以高灵敏度地用于测定人血清中的CEA。基于纸张的设备为灵敏的免疫分析和即时诊断提供了一种新的洗涤策略。
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