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Applications of the green fluorescent protein in cell biology and biotechnology.
Nature Biotechnology ( IF 33.1 ) Pub Date : 1997 Oct , DOI: 10.1038/nbt1097-961
Tom Misteli , David L. Spector

The recent emergence of an autofluorescent protein, the green fluorescent protein (GFP), has opened the door for the convenient use of intact living cells and organisms as experimental systems in fields ranging from cell biology to biomedicine. We present an overview of some of the major applications of GFP, namely its use in protein tagging and in monitoring gene expression as well as its potential in a variety of biological screens.

中文翻译:

绿色荧光蛋白在细胞生物学和生物技术中的应用。

自发荧光蛋白,绿色荧光蛋白(GFP)的最新出现为方便地使用完整的活细胞和生物体作为从细胞生物学到生物医学领域的实验系统打开了大门。我们对GFP的一些主要应用进行了概述,即其在蛋白质标签和监测基因表达中的用途以及其在各种生物学筛选中的潜力。
更新日期:2017-01-31
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