Fluorescent Photoswitchable Diarylethenes for Biolabeling and Single-Molecule Localization Microscopies with Optical Superresolution,Journal of the American Chemical Society

当前位置： X-MOL 学术 › J. Am. Chem. Soc. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Fluorescent Photoswitchable Diarylethenes for Biolabeling and Single-Molecule Localization Microscopies with Optical Superresolution
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2017-05-04 , DOI: 10.1021/jacs.7b00274
Benoît Roubinet ₁ , Michael Weber ₁ , Heydar Shojaei ₁ , Mark Bates ₁ , Mariano L. Bossi ₁ , Vladimir N. Belov ₁ , Masahiro Irie ₂ , Stefan W. Hell ₁

Affiliation

A modular assembly of water-soluble diarylethenes (DAEs), applicable as biomarkers for optical nanoscopy, is reported. Reversibly photoswitchable 1,2-bis(2-alkyl-6-phenyl-1-benzothiophene-1,1-dioxide-3-yl)perfluorocyclopentenes possessing a fluorescent "closed" form were decorated with one or two methoxy group(s) attached to the para-position(s) of phenyl ring(s) and two, four, or eight carboxylic acid groups. Antibody conjugates of these DAEs feature low aggregation, efficient photoswitching in aqueous buffers, specific staining of cellular structures, and photophysical properties (high emission efficiencies and low cycloreversion quantum yields) enabling their application in superresolution microscopy. Images of tubulin, vimentin, and nuclear pore complexes are presented. The superresolution images can also be acquired by using solely 488 nm light without additional photoactivation with UV light. These DAEs exhibit reversible photoswitching without requiring any additives to the imaging media and open new paths toward the modular design of fluorescent dyes for bioimaging with optical superresolution.

中文翻译：

用于生物标记和具有光学超分辨率的单分子定位显微镜的荧光光开关二芳基乙烯

报告了水溶性二芳基乙烯 (DAE) 的模块化组装，可用作光学纳米显微镜的生物标志物。具有荧光“闭合”形式的可逆光开关 1,2-双（2-烷基-6-苯基-1-苯并噻吩-1,1-二氧化物-3-基）全氟环戊烯装饰有一个或两个连接的甲氧基到苯环的对位和两个、四个或八个羧酸基团。这些 DAE 的抗体偶联物具有低聚集性、在水性缓冲液中的高效光开关、细胞结构的特异性染色和光物理特性（高发射效率和低循环回复量子产率），使其能够应用于超分辨率显微镜。显示了微管蛋白、波形蛋白和核孔复合物的图像。超分辨率图像也可以通过仅使用 488 nm 光而无需额外的紫外线光激活来获得。这些 DAE 表现出可逆的光开关，无需在成像介质中添加任何添加剂，并为用于具有光学超分辨率的生物成像的荧光染料的模块化设计开辟了新的途径。

更新日期：2017-05-04

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南