Eupenifeldin was isolated from cultures of Eupenicillium brefeldianum ATCC 74184 by extraction and crystallization. The compound was identified as a pentacyclic bistropolone on the basis of spectral data and its complete structure was established by single-crystal X-ray analysis. The compound is cytotoxic against the HCT-116 cell line and has in vivo antitumor activity in the P388 leukemia model.

中文翻译：

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Eupenifeldin，一种新的细胞毒性双速龙，来自于短孢大戟属（Eupenicillium brefeldianum）。

通过提取和结晶从短白屈菜小球藻ATCC 74184的培养物中分离出大叶艾菲尼丁。根据光谱数据将该化合物鉴定为五环双酮酮，并通过单晶X射线分析确定其完整结构。该化合物对HCT-116细胞系具有细胞毒性，并且在P388白血病模型中具有体内抗肿瘤活性。