Mitigating the Hook Effect in Lateral Flow Sandwich Immunoassays Using Real-Time Reaction Kinetics,Analytical Chemistry

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Mitigating the Hook Effect in Lateral Flow Sandwich Immunoassays Using Real-Time Reaction Kinetics
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-04-13 00:00:00 , DOI: 10.1021/acs.analchem.7b00638
Elizabeth G Rey ₁ , Dakota O'Dell ₂ , Saurabh Mehta _{3,

4} , David Erickson _{1,

3,

4}

Affiliation

The quantification of analyte concentrations using lateral flow assays is a low-cost and user-friendly alternative to traditional lab-based assays. However, sandwich-type immunoassays are often limited by the high-dose hook effect, which causes falsely low results when analytes are present at very high concentrations. In this paper, we present a reaction kinetics-based technique that solves this problem, significantly increasing the dynamic range of these devices. With the use of a traditional sandwich lateral flow immunoassay, a portable imaging device, and a mobile interface, we demonstrate the technique by quantifying C-reactive protein concentrations in human serum over a large portion of the physiological range. The technique could be applied to any hook effect-limited sandwich lateral flow assay and has a high level of accuracy even in the hook effect range.

中文翻译：

利用实时反应动力学减轻侧流夹心免疫测定中的钩效应

使用侧流测定对分析物浓度进行定量是传统实验室测定的一种低成本且用户友好的替代方案。然而，夹心型免疫测定通常受到高剂量钩状效应的限制，当分析物浓度非常高时，会导致错误的低结果。在本文中，我们提出了一种基于反应动力学的技术来解决这个问题，显着增加这些设备的动态范围。通过使用传统的夹心侧流免疫测定、便携式成像设备和移动界面，我们通过在大部分生理范围内量化人血清中的 C 反应蛋白浓度来演示该技术。该技术可应用于任何钩状效应限制的夹心侧流测定，并且即使在钩状效应范围内也具有很高的准确性。

更新日期：2017-04-13

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