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Development of a Rapid LC-MS/MS Method for the Quantification of Cannabidiol, Cannabidivarin, Δ9-Tetrahydrocannabivarin, and Cannabigerol in Mouse Peripheral Tissues
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-04-03 00:00:00 , DOI: 10.1021/acs.analchem.7b01094 Fabiana Piscitelli 1, 2 , Ester Pagano 2, 3 , Anna Lauritano 1, 2 , Angelo A. Izzo 2, 3 , Vincenzo Di Marzo 1, 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-04-03 00:00:00 , DOI: 10.1021/acs.analchem.7b01094 Fabiana Piscitelli 1, 2 , Ester Pagano 2, 3 , Anna Lauritano 1, 2 , Angelo A. Izzo 2, 3 , Vincenzo Di Marzo 1, 2
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Cannabis has been known as a medicine for several thousand years across many cultures and its beneficial effects are mostly due to the presence of cannabinoids, unique natural products, whose pharmacology is going to gain increasing interest in the scientific community. The discovery of the main psychoactive constituent of Cannabis sativa L., Δ9-tetrahydrocannabinol (Δ9-THC), led to the identification of at least 100 additional phytocannabinoids, including cannabidiol (CBD), cannabidivarin (CBDV), Δ9-tetrahydrocannabivarin (Δ9-THCV), and cannabigerol (CBG). These molecules are gaining growing interest for their medical properties; however, further research is needed to assess the differences in their pharmacokinetic and pharmacodymanic profiles. The aim of this study was to set up a rapid and accurate method, by using the LC-MS-IT-TOF technology, to detect and quantify CBD, CBDV, Δ9-THCV, and CBG in biological matrices. Data show that the method developed here is linear in the calibration range; recoveries from mouse tissues were in the 50–60% range and sensitivity was 2 ng/mL for CBDV, 4 ng/mL for CBG and THCV, and 7 ng/mL for CBD. The method is rapid, precise and accurate, and it will represent a fundamental tool to evaluate the pharmacokinetic and pharmacodynamic properties of selected phytocannabinoids in tissues from different animal models, and develop new cannabinoid-based medicine.
中文翻译：
为大麻二酚的定量，Cannabidivarin，Δ一快速LC-MS / MS方法的发展9 -Tetrahydrocannabivarin，和大麻萜酚小鼠外周组织
大麻在许多文化中已被称为数千年的药物，其有益作用主要是由于存在大麻素这一独特的天然产物，其药理学将在科学界引起越来越多的兴趣。的主要精神成分的发现大麻L.，Δ 9 -四氢大麻酚（Δ 9 -THC），导致至少100个附加phytocannabinoids，包括大麻二酚（CBD），cannabidivarin（CBDV）的鉴定，Δ 9 -tetrahydrocannabivarin （Δ 9-THCV）和大麻二酚（CBG）。这些分子因其医学特性而受到越来越多的关注。但是，需要进一步研究以评估其药代动力学和药代动力学特征的差异。本研究的目的是建立一个快速，准确的方法，通过使用LC-MS-IT-TOF技术，来检测和量化CBD，CBDV，Δ 9-THCV和生物基质中的CBG。数据表明，这里开发的方法在校准范围内是线性的；小鼠组织的回收率在50–60％范围内，CBDV的灵敏度为2 ng / mL，CBG和THCV的灵敏度为4 ng / mL，CBD的灵敏度为7 ng / mL。该方法快速，准确和准确，将成为评估来自不同动物模型的组织中所选植物大麻素的药代动力学和药效学性质，并开发新的基于大麻素的药物的基本工具。
更新日期：2017-04-03
中文翻译：
为大麻二酚的定量，Cannabidivarin，Δ一快速LC-MS / MS方法的发展9 -Tetrahydrocannabivarin，和大麻萜酚小鼠外周组织
大麻在许多文化中已被称为数千年的药物，其有益作用主要是由于存在大麻素这一独特的天然产物，其药理学将在科学界引起越来越多的兴趣。的主要精神成分的发现大麻L.，Δ 9 -四氢大麻酚（Δ 9 -THC），导致至少100个附加phytocannabinoids，包括大麻二酚（CBD），cannabidivarin（CBDV）的鉴定，Δ 9 -tetrahydrocannabivarin （Δ 9-THCV）和大麻二酚（CBG）。这些分子因其医学特性而受到越来越多的关注。但是，需要进一步研究以评估其药代动力学和药代动力学特征的差异。本研究的目的是建立一个快速，准确的方法，通过使用LC-MS-IT-TOF技术，来检测和量化CBD，CBDV，Δ 9-THCV和生物基质中的CBG。数据表明，这里开发的方法在校准范围内是线性的；小鼠组织的回收率在50–60％范围内，CBDV的灵敏度为2 ng / mL，CBG和THCV的灵敏度为4 ng / mL，CBD的灵敏度为7 ng / mL。该方法快速，准确和准确，将成为评估来自不同动物模型的组织中所选植物大麻素的药代动力学和药效学性质，并开发新的基于大麻素的药物的基本工具。
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