当前位置：
X-MOL 学术
›
Anal. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Pyridine: a Denaturant or Stabilizer of Spherical Nucleic Acids?
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-03-31 00:00:00 , DOI: 10.1021/acs.analchem.7b00005 Yoon Hyuck Kim 1 , Ju-Hwan Oh 1 , Abigail K. R. Lytton-Jean 2 , Jae-Seung Lee 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-03-31 00:00:00 , DOI: 10.1021/acs.analchem.7b00005 Yoon Hyuck Kim 1 , Ju-Hwan Oh 1 , Abigail K. R. Lytton-Jean 2 , Jae-Seung Lee 1
Affiliation
|
The spotlighted dual functions of pyridine as a denaturant and as a stabilizer for duplex DNA are thoroughly investigated using spherical nucleic acids (SNAs). At neutral pH, pyridine destabilizes the duplex interconnects of assembled SNAs, resulting in a gradual decrease in their melting temperature (Tm) as a function of the pyridine concentration. This result is in good agreement with the conventional role of pyridine as a powerful denaturant for free duplex DNA. On the contrary, the addition of pyridine dramatically increases the Tm of hybridized SNAs under acidic conditions, which could be a striking result of pyridine’s stabilizing effect for DNA duplex as previously suggested on the basis of the pyridine–nucleobase interactions. After comprehensive and quantitative investigation based on the analysis of the sharp melting transitions of SNAs, however, we report that, in fact, the pH increase induced by pyridine is also an essential parameter accounting for pyridine’s DNA-stabilizing effects under acidic conditions. Importantly, we prove that pyridine, particularly at a low concentration, does not increase the Tm of hybridized SNAs even under acidic conditions, if the pH increase by pyridine is corrected to maintain the same initial pH.
中文翻译：
吡啶：球形核酸的变性剂或稳定剂？
使用球形核酸（SNA）彻底研究了吡啶作为双链DNA的变性剂和稳定剂的双重功能。在中性pH下，吡啶使组装好的SNA的双链体互连不稳定，导致它们的熔融温度（T m）随吡啶浓度的变化而逐渐降低。该结果与吡啶作为游离双链体DNA的强力变性剂的常规作用很好地吻合。相反，吡啶的加入显着增加了T m酸性条件下杂交SNA的合成，这可能是吡啶对DNA双链体稳定作用的惊人结果，如先前在吡啶-核碱基相互作用的基础上所建议的那样。在基于SNA的急剧熔融转变的分析进行全面和定量研究后，我们报告说，实际上，吡啶诱导的pH升高也是说明在酸性条件下吡啶的DNA稳定作用的重要参数。重要的是，我们证明了吡啶，特别是低浓度的吡啶，即使在酸性条件下，也不能提高杂交SNA的T m，如果通过吡啶校正pH值以维持相同的初始pH值。
更新日期：2017-03-31
中文翻译：
吡啶：球形核酸的变性剂或稳定剂？
使用球形核酸（SNA）彻底研究了吡啶作为双链DNA的变性剂和稳定剂的双重功能。在中性pH下，吡啶使组装好的SNA的双链体互连不稳定，导致它们的熔融温度（T m）随吡啶浓度的变化而逐渐降低。该结果与吡啶作为游离双链体DNA的强力变性剂的常规作用很好地吻合。相反，吡啶的加入显着增加了T m酸性条件下杂交SNA的合成，这可能是吡啶对DNA双链体稳定作用的惊人结果，如先前在吡啶-核碱基相互作用的基础上所建议的那样。在基于SNA的急剧熔融转变的分析进行全面和定量研究后，我们报告说，实际上，吡啶诱导的pH升高也是说明在酸性条件下吡啶的DNA稳定作用的重要参数。重要的是，我们证明了吡啶，特别是低浓度的吡啶，即使在酸性条件下，也不能提高杂交SNA的T m，如果通过吡啶校正pH值以维持相同的初始pH值。
京公网安备 11010802027423号