多功能纤维素酶比纳米纤维素和生物燃料联产的过程性真菌纤维素酶更好,ACS Nano

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多功能纤维素酶比纳米纤维素和生物燃料联产的过程性真菌纤维素酶更好
ACS Nano ( IF 15.8 ) Pub Date : 2017-03-10 00:00:00 , DOI: 10.1021/acsnano.7b00086
John. M. Yarbrough ₁ , Ruoran Zhang ₁ , Ashutosh Mittal ₁ , Todd Vander Wall ₁ , Yannick J. Bomble ₁ , Stephen R. Decker ₁ , Michael E. Himmel ₁ , Peter N. Ciesielski ₁

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用可再生资源生产燃料，化学物质和材料以满足社会需求，这仍然是向可持续，清洁能源经济过渡的重要一步。将纤维素分解酶用于生产纳米纤维素使得能够以与现代木质纤维素（第二代）生物精炼厂所采用的工艺设计基本兼容的形式共同生产用于生物燃料的糖。然而，酶促产生的纳米纤维素的产率通常比通过无机酸产生方法获得的产率低得多。在这项研究中，我们比较了使用两种截然不同的纤维素酶系统共同生产纳米纤维素和可发酵糖的能力：腐生真菌里氏木霉（T. reesei）的经典“游离酶”系统）和温泉居民Caldicellulosiruptor bescii（C. bescii）产生的复杂的多功能酶。通过比较消化，我们证明了贝氏梭菌系统在总纤维素转化率，糖产量和纳米纤维素产量方面优于真菌酶体系。另外，我们通过多峰成像和动态光散射显示，由贝氏梭菌纤维素酶系统产生的纳米纤维素比里氏木霉产生的纳米纤维素实质上更均匀。系统。这些不同的系统所产生的纳米纤维素的产量和特性上的这些差异，可以归因于每个系统中主要酶的作用机理的巨大差异。

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Multifunctional Cellulolytic Enzymes Outperform Processive Fungal Cellulases for Coproduction of Nanocellulose and Biofuels

Producing fuels, chemicals, and materials from renewable resources to meet societal demands remains an important step in the transition to a sustainable, clean energy economy. The use of cellulolytic enzymes for the production of nanocellulose enables the coproduction of sugars for biofuels production in a format that is largely compatible with the process design employed by modern lignocellulosic (second generation) biorefineries. However, yields of enzymatically produced nanocellulose are typically much lower than those achieved by mineral acid production methods. In this study, we compare the capacity for coproduction of nanocellulose and fermentable sugars using two vastly different cellulase systems: the classical “free enzyme” system of the saprophytic fungus, Trichoderma reesei (T. reesei) and the complexed, multifunctional enzymes produced by the hot springs resident, Caldicellulosiruptor bescii (C. bescii). We demonstrate by comparative digestions that the C. bescii system outperforms the fungal enzyme system in terms of total cellulose conversion, sugar production, and nanocellulose production. In addition, we show by multimodal imaging and dynamic light scattering that the nanocellulose produced by the C. bescii cellulase system is substantially more uniform than that produced by the T. reesei system. These disparities in the yields and characteristics of the nanocellulose produced by these disparate systems can be attributed to the dramatic differences in the mechanisms of action of the dominant enzymes in each system.

更新日期：2017-03-10

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