Polymer Nanoparticle Engineering for Podocyte Repair: From in Vitro Models to New Nanotherapeutics in Kidney Diseases,ACS Omega

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Polymer Nanoparticle Engineering for Podocyte Repair: From in Vitro Models to New Nanotherapeutics in Kidney Diseases
ACS Omega ( IF 3.7 ) Pub Date : 2017-02-20 00:00:00 , DOI: 10.1021/acsomega.6b00423
Claudio Colombo ₁ , Min Li _{2,

3} , Shojiro Watanabe ₃ , Piergiorgio Messa ₃ , Alberto Edefonti ₄ , Giovanni Montini ₄ , Davide Moscatelli ₁ , Maria Pia Rastaldi ₃ , Francesco Cellesi _{1,

2,

3}

Affiliation

Specific therapeutic targeting of kidney podocytes, the highly differentiated ramified glomerular cells involved in the onset and/or progression of proteinuric diseases, could become the optimal strategy for preventing chronic kidney disease. With this aim, we developed a library of engineered polymeric nanoparticles (NPs) of tuneable size and surface properties and evaluated their interaction with podocytes. NP cytotoxicity, uptake, and cytoskeletal effects on podocytes were first assessed. On the basis of these data, nanodelivery of dexamethasone loaded into selected biocompatible NPs was successful in repairing damaged podocytes. Finally, a three-dimensional in vitro system of co-culture of endothelial cells and podocytes was exploited as a new tool for mimicking the mechanisms of NP interaction with glomerular cells and the repair of the kidney filtration barrier.

中文翻译：

用于足细胞修复的聚合物纳米颗粒工程：从体外模型到肾脏疾病的新型纳米治疗

肾足细胞的特异性治疗靶点是参与蛋白尿疾病发作和/或发展的高度分化的分支肾小球细胞，可能成为预防慢性肾脏疾病的最佳策略。为此，我们开发了可调整大小和表面特性的工程聚合纳米颗粒（NP）库，并评估了它们与足细胞的相互作用。首先评估NP对足细胞的细胞毒性，摄取和细胞骨架作用。根据这些数据，将地塞米松的纳米递送装载到选定的生物相容性NP中可以成功修复受损的足细胞。最后，

更新日期：2017-02-20

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