<br>产 H2 古菌 Thermococcus onnurineus NA1 的全基因组初级转录组分析。,Scientific Reports

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产 H2 古菌 Thermococcus onnurineus NA1 的全基因组初级转录组分析。
Scientific Reports ( IF 3.8 ) Pub Date : 2017-02-20 , DOI: 10.1038/srep43044
Suhyung Cho ₁ , Min-Sik Kim ₂ , Yujin Jeong ₁ , Bo-Rahm Lee ₃ , Jung-Hyun Lee ₂ , Sung Gyun Kang ₂ , Byung-Kwan Cho _{1,

3}

Affiliation

尽管古菌基因组在转录和转录后过程中发挥着关键作用，但其调控元件尚未完全了解。在这里，我们确定了产生H ₂的古菌Thermococcus onnurineus NA1 的主要转录组。我们鉴定了 1,082 个富含嘌呤的转录起始位点，以及启动子区域中保守的 TATA 盒、富含 A 的 B 识别元件 (BRE) 和启动子近端元件 (PPE) 基序，以及高嘧啶核苷酸含量 (T/C) -1 位，以及 5' 非翻译区 (5' UTR) 中的 Shine-Dalgarno (SD) 基序 (GGDGRD)。除了差异转录水平外，还鉴定了 117 个无前导基因和 86 个非编码 RNA (ncRNA)，代表不同生长条件下的不同细胞功能和潜在调节功能。有趣的是，我们观察到 ncRNA 中的 GC 含量较低，通过非结构化形式或与其他细胞成分的相互作用进行基于 RNA 的调节。对T. onnurineus上游调控序列与密切相关的古菌基因组上游调控序列的进一步比较分析表明，直系同源基因的转录是由高度保守的启动子序列启动的，然而它们的转录和翻译调控的上游序列在很大程度上是不同的。这些结果为其未来在代谢工程中的应用提供了T. onnurineus的遗传信息。

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Genome-wide primary transcriptome analysis of H2-producing archaeon Thermococcus onnurineus NA1.

In spite of their pivotal roles in transcriptional and post-transcriptional processes, the regulatory elements of archaeal genomes are not yet fully understood. Here, we determine the primary transcriptome of the H₂-producing archaeon Thermococcus onnurineus NA1. We identified 1,082 purine-rich transcription initiation sites along with well-conserved TATA box, A-rich B recognition element (BRE), and promoter proximal element (PPE) motif in promoter regions, a high pyrimidine nucleotide content (T/C) at the -1 position, and Shine-Dalgarno (SD) motifs (GGDGRD) in 5' untranslated regions (5' UTRs). Along with differential transcript levels, 117 leaderless genes and 86 non-coding RNAs (ncRNAs) were identified, representing diverse cellular functions and potential regulatory functions under the different growth conditions. Interestingly, we observed low GC content in ncRNAs for RNA-based regulation via unstructured forms or interaction with other cellular components. Further comparative analysis of T. onnurineus upstream regulatory sequences with those of closely related archaeal genomes demonstrated that transcription of orthologous genes are initiated by highly conserved promoter sequences, however their upstream sequences for transcriptional and translational regulation are largely diverse. These results provide the genetic information of T. onnurineus for its future application in metabolic engineering.

更新日期：2017-02-21

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