It is significant to develop probes for rapid, selective, and sensitive detection of highly toxic hydrazine in both environmental and biological science. In this work, under mild conditions, through the electrostatic attraction between negatively charged CDs and positively charged hemicyanine molecules, a novel ratiometric fluorescent probe containing CDs and a hemicyanine derivative was fabricated for reliable, selective, and sensitive sensing of hydrazine. This nanohybrid system possesses dual emission peaks at 550 and 610 nm under a single excitation wavelength of 530 nm. The addition of hydrazine to CDs–hemicyanine nanohybrid solution results in complete fluorescence quenching of the hemicyanine derivative, while the orange fluorescence of CDs remains constant. Furthermore, the CDs–hemicyanine nanohybrid system shows high selectivity toward hydrazine over other various species, including some nucleophilic species, metal ions and anions. The limit of quantification (LOQ) was 0–1 mM and the detection limit was as low as 8.0 μM.

中文翻译：

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碳点-半花菁纳米杂化体系的比重发光检测†

在环境和生物科学中，开发用于快速，选择性和灵敏检测高毒性肼的探针具有重要意义。在这项工作中，在温和的条件下，通过带负电荷的CD与带正电荷的半菁分子之间的静电吸引，制造了一种包含CD和半菁衍生物的新型配比荧光探针，用于可靠，选择性和灵敏地检测肼。该纳米杂化系统在530 nm的单个激发波长下在550和610 nm处具有双发射峰。向CD-半花菁纳米混合溶液中添加肼可导致半菁衍生物完全荧光猝灭，而CD的橙色荧光保持恒定。此外，CDs-半花菁纳米杂化体系对肼的选择性高于其他各种物种，包括一些亲核物种，金属离子和阴离子。定量限（LOQ）为0-1 mM，检测限低至8.0μM。