A novel Foster resonance energy transfer (FRET) immunoassay based on persistent luminescence nanoparticles (PLNP) was established for prostate specific antigen (PSA) detection in serum and cell extracts without in situ excitation. The specific FRET behavior allows highly selective and sensitive ratiometric photoluminescent detection of PSA in biological samples.

中文翻译：

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生物共轭持续发光纳米颗粒，无需原位激发，即可在血清和细胞提取物中进行前列腺特异性抗原的Foster共振能量转移免疫测定。

建立了一种基于持久发光纳米粒子（PLNP）的新型Foster共振能量转移（FRET）免疫测定法，用于在无原位激发的情况下检测血清和细胞提取物中的前列腺特异性抗原（PSA）。特定的FRET行为允许对生物样品中的PSA进行高度选择性和灵敏的比例光致发光检测。