Diverse fungal endophytes are rich fungal resources for the production of an enormous quantity of natural products. In the present study, 53 fungal endophytes were isolated from the bulbs of Fritillaria unibracteata var. wabuensis (FUW). Of these, 49 strains were identified and grouped into 17 different taxa, and priority was conferred to the Fusarium genus. All fungal fermented filtrates displayed antioxidant activities. The DPPH activity, total antioxidant capacities (ABTS), reduction power (FRAP), total phenolic content (TPC), total flavonoid content (TFC) and total saponin content (TSC) were evaluated using petroleum ether, ethyl acetate, n-butyl alcohol and ethanol fractions extracted from five representative fungal cultures. The last three fractions showed more potent antioxidant activity than the first fraction. Significant positive correlations were found between the compositions (TPC, TFC and TSC) and antioxidant capacities (DPPH, ABTS and FRAP). In addition, multifarious natural antioxidant components were identified from the fungal extracts, including gallic acid, rutin, phlorizin, 2,4-di-tert-butylphenol and 2,6-di-tert-butyl hydroquinone; these were determined preliminarily by TLC-bioautography, HPLC and GC-MS analysis. This study showed abundant fungal resources in FUW. Phenolics, flavonoids and saponins are crucial bioactive constituents in these abundant fungal endophytes and can be viewed as new potential antioxidant resources.

中文翻译：

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真菌内生真菌衍生的贝母 wabuensis：多样性，体外抗氧化能力以及与酚类，类黄酮或皂苷化合物的关系。

多样的真菌内生菌是丰富的真菌资源，可用于生产大量的天然产物。在本研究中，从贝母贝的鳞茎中分离出53种真菌内生菌。wabuensis（FUW）。其中，鉴定出49个菌株并将其分为17个不同的分类单元，镰刀菌属被赋予优先权。所有真菌发酵滤液均显示出抗氧化活性。使用石油醚，乙酸乙酯，正丁醇评估了DPPH活性，总抗氧化剂能力（ABTS），还原能力（FRAP），总酚含量（TPC），总类黄酮含量（TFC）和总皂苷含量（TSC）从五种代表性真菌培养物中提取的乙醇和乙醇馏分。后三个馏分显示出比第一馏分更有效的抗氧化活性。在组成（TPC，TFC和TSC）和抗氧化剂容量（DPPH，ABTS和FRAP）之间发现了显着的正相关。此外，从真菌提取物中鉴定出多种天然抗氧化剂成分，包括没食子酸，芦丁，苯并二氮杂，2,4-二叔丁基苯酚和2,6-二叔丁基对苯二酚。这些是通过TLC-生物自传术，HPLC和GC-MS分析初步确定的。这项研究显示了FUW中丰富的真菌资源。酚类，类黄酮和皂苷是这些丰富的真菌内生植物中至关重要的生物活性成分，可以被视为新的潜在抗氧化剂资源。包括没食子酸，芦丁，草皮锌，2,4-二叔丁基苯酚和2,6-二叔丁基对苯二酚; 这些是通过TLC-生物自传术，HPLC和GC-MS分析初步确定的。这项研究显示了FUW中丰富的真菌资源。酚类，类黄酮和皂苷是这些丰富的真菌内生植物中至关重要的生物活性成分，可以被视为新的潜在抗氧化剂资源。包括没食子酸，芦丁，草皮锌，2,4-二叔丁基苯酚和2,6-二叔丁基对苯二酚; 这些是通过TLC-生物自传术，HPLC和GC-MS分析初步确定的。这项研究显示了FUW中丰富的真菌资源。酚类，类黄酮和皂苷是这些丰富的真菌内生植物中至关重要的生物活性成分，可以被视为新的潜在抗氧化剂资源。