胶体渗透压的跨膜差异影响脂质体包裹浓缩蛋白溶液的脂质膜流动性。,Langmuir

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胶体渗透压的跨膜差异影响脂质体包裹浓缩蛋白溶液的脂质膜流动性。
Langmuir ( IF 3.7 ) Pub Date : 2017-01-31 00:00:00 , DOI: 10.1021/acs.langmuir.6b04643
Tomoko Kure ₁ , Hiromi Sakai ₁

Affiliation

血红蛋白囊泡（Hb-V）是将高浓度的血红蛋白溶液（40 g / dL）包裹在脂质体中的人造氧气载体。广泛研究了Hb-V悬浮液作为输血替代品的体内安全性和有效性以及在储存过程中的结构稳定性。由于脂质体内的血红蛋白水溶液所具有的胶体渗透压（COP，200–300 Torr）远高于血浆（20–25 Torr），因此脂质膜是否能感应到COP的跨膜差异成为一个问题。我们使用荧光偏振技术检查了膜的微粘度。为了避免红色Hb干扰荧光测量，我们使用人血清白蛋白（HSA）替代Hb。HSA和Hb溶液均显示高COP，具体取决于浓度。将HSA溶液（40 g / dL）封装在脂质体中会降低在较低温度（25°C时为949±8 cP→607±10 cP）的膜微粘度。结果表明，HSA包埋引起的跨膜渗透应力随着球形表面积的增加而最大程度地扩展脂质体，并且膜的流动性大大增加。即使在这种情况下，最低的膜微粘度在60°C时为377±10 cP，也远高于1,2-二棕榈酰-Sn-甘油-3-磷脂酰胆碱脂质体（60°C时40±2 cP）。因此，如文献所述，Hb-V以及HSA-V在由高COP引起的跨膜应力下保持球形结构和机械稳定性。

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Transmembrane Difference in Colloid Osmotic Pressure Affects the Lipid Membrane Fluidity of Liposomes Encapsulating a Concentrated Protein Solution

A hemoglobin vesicle (Hb-V) is an artificial oxygen carrier encapsulating a highly concentrated hemoglobin solution (40 g/dL) in a liposome. The in vivo safety and efficacy of Hb-V suspension as a transfusion alternative and structural stability during storage have been studied extensively. Because the intraliposomal Hb aqueous solution can possess colloid osmotic pressure (COP, 200–300 Torr) that is much higher than that of blood plasma (20–25 Torr), a question arises as to whether the lipid membrane senses the transmembrane difference in COP. We examined the membrane microviscosity using a fluorescence polarization technique. To avoid the interference of red Hb on the fluorescence measurement, we used human serum albumin (HSA) as a substitute for Hb. Both HSA and Hb solutions show high COP depending on the concentration. Encapsulation of HSA solution (40 g/dL) in the liposome decreased the membrane microviscosity at a lower temperature (949 ± 8 cP → 607 ± 10 cP at 25 °C). The result indicates that the transmembrane osmotic stress induced by HSA encapsulation expands the liposome maximally with increasing spherical surface area, and the membrane fluidity is increased extremely. Even for such a condition, the lowest membrane microviscosity, 377 ± 10 cP at 60 °C, is much higher than that of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine liposome (40 ± 2 cP at 60 °C). Accordingly, Hb-V as well as HSA-V maintains a spherical structure and mechanical stability under transmembrane stress caused by high COP, as described in the literature.

更新日期：2017-01-31

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