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Construction of AIEgens-Based Bioprobe with Two Fluorescent Signals for Enhanced Monitor of Extracellular and Intracellular Telomerase Activity
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-01-27 00:00:00 , DOI: 10.1021/acs.analchem.6b04696
Yuan Zhuang 1 , Chunli Shang 1 , Xiaoding Lou 1 , Fan Xia 1, 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-01-27 00:00:00 , DOI: 10.1021/acs.analchem.6b04696
Yuan Zhuang 1 , Chunli Shang 1 , Xiaoding Lou 1 , Fan Xia 1, 2
Affiliation
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Detections of telomerase activity in vitro and in living cells are of great importance for clinical diagnosis of cancer. In this work, an AIEgens-based bioprobe with two fluorescent signals for enhanced monitor of extracellular and intracellular telomerase activity is designed. After addition of telomerase, two positively charged AIEgens (Silole-R and TPE-H) bind to quencher group labeled primer (QP) and the extension repeated units, leading enhancement of two telomerase-triggered fluorescent signals. Furthermore, by combination the wider linear range in vitro and lower background in living cells imaging, the bioprobe is used to detect telomerase extracted from various cell lines (MCF-7, HeLa, E-J, and HLF), 50 bladder cancer patients’ urine samples, 10 normal people’s urine samples, and also applied in mapping telomerase activity inside living cells (MCF-7, HeLa, MDA-MB-231, and HT1080). The results show that this well-designed strategy can successfully detect telomerase activity in vitro and in living cells with high sensitivity, indicating the potential application of this method in cancer cells bioimaging and clinical cancer diagnosis.
中文翻译：
具有两个荧光信号的增强的细胞外和细胞内端粒酶活性监测的基于AIEgens的生物探针的构建
体外和活细胞中端粒酶活性的检测对于癌症的临床诊断非常重要。在这项工作中，基于AIEgens的生物探针具有两个荧光信号，用于增强对细胞外和细胞内端粒酶活性的监测。添加端粒酶后，两个带正电荷的AIEgen（Silole-R和TPE-H）与淬灭剂组标记的引物（QP）和延伸重复单元结合，导致两个端粒酶触发的荧光信号增强。此外，通过结合更宽的体外线性范围和较低的活细胞成像背景，该生物探针可用于检测从各种细胞系（MCF-7，HeLa，EJ和HLF），50个膀胱癌患者尿液样本中提取的端粒酶，10个正常人的尿液样本，还可用于定位活细胞（MCF-7，HeLa，MDA-MB-231和HT1080）内部的端粒酶活性。结果表明，这种精心设计的策略可以在体外和活细胞中以高灵敏度成功检测端粒酶活性，表明该方法在癌细胞生物成像和临床癌症诊断中的潜在应用。
更新日期：2017-01-27
中文翻译：
具有两个荧光信号的增强的细胞外和细胞内端粒酶活性监测的基于AIEgens的生物探针的构建
体外和活细胞中端粒酶活性的检测对于癌症的临床诊断非常重要。在这项工作中，基于AIEgens的生物探针具有两个荧光信号，用于增强对细胞外和细胞内端粒酶活性的监测。添加端粒酶后，两个带正电荷的AIEgen（Silole-R和TPE-H）与淬灭剂组标记的引物（QP）和延伸重复单元结合，导致两个端粒酶触发的荧光信号增强。此外，通过结合更宽的体外线性范围和较低的活细胞成像背景，该生物探针可用于检测从各种细胞系（MCF-7，HeLa，EJ和HLF），50个膀胱癌患者尿液样本中提取的端粒酶，10个正常人的尿液样本，还可用于定位活细胞（MCF-7，HeLa，MDA-MB-231和HT1080）内部的端粒酶活性。结果表明，这种精心设计的策略可以在体外和活细胞中以高灵敏度成功检测端粒酶活性，表明该方法在癌细胞生物成像和临床癌症诊断中的潜在应用。
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