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Porphyrin-Based Porous Organic Frameworks as a Biomimetic Catalyst for Highly Efficient Colorimetric Immunoassay
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2017-01-18 00:00:00 , DOI: 10.1021/acsami.6b15637
Xiao Deng 1 , Yishan Fang 2 , Sha Lin 1 , Qian Cheng 1 , Qingyun Liu 3 , Xiaomei Zhang 1
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2017-01-18 00:00:00 , DOI: 10.1021/acsami.6b15637
Xiao Deng 1 , Yishan Fang 2 , Sha Lin 1 , Qian Cheng 1 , Qingyun Liu 3 , Xiaomei Zhang 1
Affiliation
Herein, we synthesized a cost-effective iron porphyrin (FePor)-based covalent organic polymer (COP), FePor-TFPA-COP, through an easy aromatic substitution reaction between pyrrole and tris(4-formylphenyl)amine (TFPA). The triangular pyramid-shaped, N-centric structure of TFPA facilitated the formation of FePor-TFPA-COP with three-dimensional porous structure, larger surface area, and abundant surface catalytically active sites. FePor-TFPA-COP exhibited strong intrinsic peroxidase activity toward a classical peroxidase substrate, 3,3′,5,5′-tetramethylbenzidine (TMB), in the presence of H2O2. Compared with horseradish peroxidase (HRP), FePor-TFPA-COP exhibited several advantages such as easy storage, high sensitivity, and prominently chemical and catalytic stability under the harsh conditions, which guaranteed the accuracy and reliability of measurements. Utilizing the excellent catalytic activity, a FePor-TFPA-COP-based colorimetric immunoassay was first established for α-fetoprotein (AFP) detection and showed high sensitivity, stability, and acceptable reproducibility. The linear response range for AFP was 5 pg/mL to 100 ng/mL and the detection limitation was 1 pg/mL. The routine provided a brilliant biomimetic catalyst to develop the nonenzyme immunoassay. More importantly, the high chemical and catalytic stability and sensitivity facilitated future practical applications under various conditions.
中文翻译:
基于卟啉的多孔有机骨架作为高效仿色免疫测定的仿生催化剂
在本文中,我们通过吡咯与三(4-甲酰基苯基)胺(TFPA)之间的易芳族取代反应,合成了具有成本效益的基于卟啉铁(FePor)的共价有机聚合物(COP)FePor-TFPA-COP。TFPA的三角形金字塔形,N中心结构有利于FePor-TFPA-COP的形成,具有三维多孔结构,较大的表面积和丰富的表面催化活性位。在存在H 2 O 2的情况下,FePor-TFPA-COP对经典的过氧化物酶底物3,3',5,5'-四甲基联苯胺(TMB)表现出很强的固有过氧化物酶活性。。与辣根过氧化物酶(HRP)相比,FePor-TFPA-COP具有多种优点,例如易于存储,灵敏度高以及在苛刻条件下的突出的化学和催化稳定性,从而确保了测量的准确性和可靠性。利用出色的催化活性,首次建立了基于FePor-TFPA-COP的比色免疫分析法用于α-甲胎蛋白(AFP)检测,并显示出高灵敏度,稳定性和可接受的重现性。AFP的线性响应范围为5 pg / mL至100 ng / mL,检测极限为1 pg / mL。该程序为开发非酶免疫分析提供了出色的仿生催化剂。更重要的是,高的化学和催化稳定性和敏感性促进了未来在各种条件下的实际应用。
更新日期:2017-01-18
中文翻译:
基于卟啉的多孔有机骨架作为高效仿色免疫测定的仿生催化剂
在本文中,我们通过吡咯与三(4-甲酰基苯基)胺(TFPA)之间的易芳族取代反应,合成了具有成本效益的基于卟啉铁(FePor)的共价有机聚合物(COP)FePor-TFPA-COP。TFPA的三角形金字塔形,N中心结构有利于FePor-TFPA-COP的形成,具有三维多孔结构,较大的表面积和丰富的表面催化活性位。在存在H 2 O 2的情况下,FePor-TFPA-COP对经典的过氧化物酶底物3,3',5,5'-四甲基联苯胺(TMB)表现出很强的固有过氧化物酶活性。。与辣根过氧化物酶(HRP)相比,FePor-TFPA-COP具有多种优点,例如易于存储,灵敏度高以及在苛刻条件下的突出的化学和催化稳定性,从而确保了测量的准确性和可靠性。利用出色的催化活性,首次建立了基于FePor-TFPA-COP的比色免疫分析法用于α-甲胎蛋白(AFP)检测,并显示出高灵敏度,稳定性和可接受的重现性。AFP的线性响应范围为5 pg / mL至100 ng / mL,检测极限为1 pg / mL。该程序为开发非酶免疫分析提供了出色的仿生催化剂。更重要的是,高的化学和催化稳定性和敏感性促进了未来在各种条件下的实际应用。