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DNA Modified Fe3O4@Au Magnetic Nanoparticles as Selective Probes for Simultaneous Detection of Heavy Metal Ions
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2017-01-23 00:00:00 , DOI: 10.1021/acsami.6b14247 Peng Miao 1, 2 , Yuguo Tang 1, 2 , Lei Wang 1
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2017-01-23 00:00:00 , DOI: 10.1021/acsami.6b14247 Peng Miao 1, 2 , Yuguo Tang 1, 2 , Lei Wang 1
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Driven by the urgent need to detect trace heavy metal ions in various real water samples, this article demonstrates for the first time an electrochemical biosensor based on DNA modified Fe3O4@Au magnetic nanoparticles (NPs). Three DNA probes are designed to contain certain mismatched base pairs. One is thiolated and modified on the surface of Fe3O4@Au NPs (DNA 1). The other two probes (DNA 2 and 3) are labeled with two independent electrochemical species. Stable structures of cytosine–Ag+–cytosine and thymine–Hg2+–thymine formed in the presence of Ag+ and Hg2+ can assist the hybridization of DNA 1/DNA 2 and DNA 1/DNA 3, which locate corresponding electrochemical species onto the surface of the magnetic NPs. The achieved nanocomposites are then used as selective electrochemical probes for the detection of heavy metal ions by recording the square wave voltammetry signals. Simultaneous detection of Ag+ and Hg2+ is demonstrated without significant interference, and their individual high sensitivities are fundamentally preserved, which meet the requirements of U.S. Environmental Protection Agency (USEPA). Furthermore, the proposed method has been challenged by various real water samples. The results confirm the DNA modified magnetic NPs based sensing method may have potential applications for the monitoring of heavy metal ions in real sample analysis.
中文翻译：
DNA修饰的Fe 3 O 4 @Au磁性纳米粒子作为同时检测重金属离子的选择性探针
迫切需要检测各种真实水样中的痕量重金属离子，本文首次展示了一种基于DNA修饰的Fe 3 O 4 @Au磁性纳米颗粒（NPs）的电化学生物传感器。设计了三个DNA探针以包含某些错配的碱基对。一种是在Fe 3 O 4 @Au NPs（DNA 1）的表面上被硫醇化和修饰的。其他两个探针（DNA 2和3）标记有两个独立的电化学物质。胞嘧啶-银的稳定结构+ -胞嘧啶和胸腺嘧啶的汞2+ -胸腺嘧啶形成在Ag的存在+和Hg 2+可以帮助DNA 1 / DNA 2和DNA 1 / DNA 3杂交，这将相应的电化学物质定位在磁性NP的表面上。然后将获得的纳米复合材料用作选择性电化学探针，通过记录方波伏安信号来检测重金属离子。同时检测Ag +和Hg 2+被证明没有明显干扰，并且从根本上保留了它们各自的高灵敏度，符合美国环境保护局（USEPA）的要求。此外，所提出的方法已经受到各种真实水样的挑战。结果证实，基于DNA修饰的磁性NPs的传感方法在实际样品分析中可能具有监测重金属离子的潜在应用。
更新日期：2017-01-23
中文翻译：
DNA修饰的Fe 3 O 4 @Au磁性纳米粒子作为同时检测重金属离子的选择性探针
迫切需要检测各种真实水样中的痕量重金属离子，本文首次展示了一种基于DNA修饰的Fe 3 O 4 @Au磁性纳米颗粒（NPs）的电化学生物传感器。设计了三个DNA探针以包含某些错配的碱基对。一种是在Fe 3 O 4 @Au NPs（DNA 1）的表面上被硫醇化和修饰的。其他两个探针（DNA 2和3）标记有两个独立的电化学物质。胞嘧啶-银的稳定结构+ -胞嘧啶和胸腺嘧啶的汞2+ -胸腺嘧啶形成在Ag的存在+和Hg 2+可以帮助DNA 1 / DNA 2和DNA 1 / DNA 3杂交，这将相应的电化学物质定位在磁性NP的表面上。然后将获得的纳米复合材料用作选择性电化学探针，通过记录方波伏安信号来检测重金属离子。同时检测Ag +和Hg 2+被证明没有明显干扰，并且从根本上保留了它们各自的高灵敏度，符合美国环境保护局（USEPA）的要求。此外，所提出的方法已经受到各种真实水样的挑战。结果证实，基于DNA修饰的磁性NPs的传感方法在实际样品分析中可能具有监测重金属离子的潜在应用。
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