Make it a double: Complementary probes with two pyrene labels were engineered for the amplification of a target DNA sequence. In the stem‐closed conformation of the probes in the absence of the target, the two pyrene moieties were separated. The target propagated hybridization chain reactions to bring pyrene moieties on neighboring probes close together to form fluorescent pyrene excimers (see picture).

中文翻译：

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基于杂交链反应的态准分子探针用于复杂生物流体中核酸的检测

使其成倍增加：设计了带有两个pyr标记的互补探针，用于扩增目标DNA序列。在不存在靶标的情况下，探针的茎为闭合构象，两个pyr部分被分离了。靶标传播了杂交链反应，使相邻探针上的pyr部分紧密靠近，形成了荧光pyr准分子（参见图片）。