Single‐Molecule Kinetics of Two‐Step Divalent Cation Chelation,Angewandte Chemie International Edition

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Single‐Molecule Kinetics of Two‐Step Divalent Cation Chelation
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2010-06-22 , DOI: 10.1002/anie.200906601
Anne F Hammerstein ₁ , Seong-Ho Shin , Hagan Bayley

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A double hug: Two half‐chelating ligands were covalently attached within the lumen of a protein nanopore (see picture). By electrical recording, the formation of fully chelated Zn²⁺ ions was monitored at the single‐molecule level, thus revealing the rate constants for the eight major steps in the process.