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Constant-Distance Mode Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging of Biological Samples with Complex Topography
Analytical Chemistry ( IF 6.7 ) Pub Date : 2016-12-28 00:00:00 , DOI: 10.1021/acs.analchem.6b03293
Son N. Nguyen 1 , Andrey V. Liyu 1 , Rosalie K. Chu 1 , Christopher R. Anderton 1 , Julia Laskin 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2016-12-28 00:00:00 , DOI: 10.1021/acs.analchem.6b03293
Son N. Nguyen 1 , Andrey V. Liyu 1 , Rosalie K. Chu 1 , Christopher R. Anderton 1 , Julia Laskin 1
Affiliation
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A new approach for constant-distance mode mass spectrometry imaging (MSI) of biological samples using nanospray desorption electrospray ionization (nano-DESI) was developed by integrating a shear-force probe with the nano-DESI probe. The technical concept and basic instrumental setup, as well as the general operation of the system are described. Mechanical dampening of resonant oscillations due to the presence of shear forces between the probe and the sample surface enabled the constant-distance imaging mode via a computer-controlled closed-feedback loop. The capability of simultaneous chemical and topographic imaging of complex biological samples is demonstrated using living Bacillus subtilis ATCC 49760 colonies on agar plates. The constant-distance mode nano-DESI MSI enabled imaging of many metabolites, including nonribosomal peptides (surfactin, plipastatin, and iturin) on the surface of living bacterial colonies, ranging in diameter from 10 to 13 mm, with height variations up to 0.8 mm above the agar plate. Co-registration of ion images to topographic images provided higher-contrast images. Based on this effort, constant-mode nano-DESI MSI proved to be ideally suited for imaging biological samples of complex topography in their native states.
中文翻译:
具有复杂形貌的生物样品的等距模式纳米喷雾解吸电喷雾电离质谱成像
通过将剪切力探针与纳米DESI探针集成在一起,开发了一种使用纳米喷雾解吸电喷雾电离(nano-DESI)对生物样品进行等距模式质谱成像(MSI)的新方法。描述了技术概念和基本的仪器设置,以及系统的一般操作。由于探头和样品表面之间存在剪切力而导致的共振振荡的机械衰减,通过计算机控制的闭环反馈回路实现了恒定距离成像模式。使用活枯草芽孢杆菌证明了同时进行复杂生物样品的化学和地形成像的能力琼脂平板上有ATCC 49760菌落。恒定距离模式的纳米DESI MSI使活细菌菌落表面上许多代谢物(包括非核糖体肽(表面活性素,普伐他汀和伊图林))成像,直径范围为10到13 mm,高度变化最大为0.8 mm在琼脂平板上。离子图像与形貌图像的共配准可提供更高对比度的图像。基于这一努力,恒定模式纳米DESI MSI被证明非常适合在原始状态下对复杂地形的生物样品进行成像。
更新日期:2016-12-28
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
具有复杂形貌的生物样品的等距模式纳米喷雾解吸电喷雾电离质谱成像
通过将剪切力探针与纳米DESI探针集成在一起,开发了一种使用纳米喷雾解吸电喷雾电离(nano-DESI)对生物样品进行等距模式质谱成像(MSI)的新方法。描述了技术概念和基本的仪器设置,以及系统的一般操作。由于探头和样品表面之间存在剪切力而导致的共振振荡的机械衰减,通过计算机控制的闭环反馈回路实现了恒定距离成像模式。使用活枯草芽孢杆菌证明了同时进行复杂生物样品的化学和地形成像的能力琼脂平板上有ATCC 49760菌落。恒定距离模式的纳米DESI MSI使活细菌菌落表面上许多代谢物(包括非核糖体肽(表面活性素,普伐他汀和伊图林))成像,直径范围为10到13 mm,高度变化最大为0.8 mm在琼脂平板上。离子图像与形貌图像的共配准可提供更高对比度的图像。基于这一努力,恒定模式纳米DESI MSI被证明非常适合在原始状态下对复杂地形的生物样品进行成像。