<br>与无规共聚物相比，区域规则交替聚两性电解质具有增强的仿生冰重结晶活性以及侧链与主链疏水性的作用,Biomacromolecules

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与无规共聚物相比，区域规则交替聚两性电解质具有增强的仿生冰重结晶活性以及侧链与主链疏水性的作用
Biomacromolecules ( IF 5.5 ) Pub Date : 2016-12-23 00:00:00 , DOI: 10.1021/acs.biomac.6b01691
Christopher Stubbs ₁ , Julia Lipecki ₁ , Matthew I Gibson ₁

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来自极地鱼类的抗冻蛋白是有效的冰重结晶抑制剂 (IRIs)，可有效阻止所有冰的生长。具有 IRI 活性的添加剂已被证明可以增强细胞冷冻保存，并有可能改善供体细胞和组织的分布。聚两性电解质是同时具有阴离子和阳离子侧链的聚合物，是一类快速新兴的聚合物冷冻保护剂，但其作用模式和活性所必需的结构特征尚不清楚。这里，区域规整性聚两性电解质由马来酸酐共聚物合成，以实现带电基团的化学计量安装，确保区域规整性，这是使用传统无规共聚不可能实现的。采用模块化合成策略来改变主链和侧链的疏水性，发现侧链疏水性对 IRI 活性有深远的影响。发现区域规整聚合物的活性优于通过单体统计掺入的标准无规共聚衍生的活性，表明序列组成对于 IRI 活性聚两性电解质的活性至关重要。

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Regioregular Alternating Polyampholytes Have Enhanced Biomimetic Ice Recrystallization Activity Compared to Random Copolymers and the Role of Side Chain versus Main Chain Hydrophobicity

Antifreeze proteins from polar fish species are potent ice recrystallization inhibitors (IRIs) effectively stopping all ice growth. Additives that have IRI activity have been shown to enhance cellular cryopreservation with potential to improve the distribution of donor cells and tissue. Polyampholytes, polymers with both anionic and cationic side chains, are a rapidly emerging class of polymer cryoprotectants, but their mode of action and the structural features essential for activity are not clear. Here regioregular polyampholytes are synthesized from maleic anhydride copolymers to enable stoichiometric installation of the charged groups, ensuring regioregularity, which is not possible using conventional random copolymerization. A modular synthetic strategy is employed to enable the backbone and side chain hydrophobicity to be varied, with side chain hydrophobicity found to have a profound effect on the IRI activity. The activity of the regioregular polymers was found to be superior to those derived from a standard random copolymerization with statistical incorporation of monomers, demonstrating that sequence composition is crucial to the activity of IRI active polyampholytes.

更新日期：2016-12-23

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