当前位置：
X-MOL 学术
›
ACS Chem. Biol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
The GLO1 Gene Is Required for Full Activity of O-Acetyl Homoserine Sulfhydrylase Encoded by MET17
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2016-12-22 00:00:00 , DOI: 10.1021/acschembio.6b00815
Matias I. Kinzurik 1 , Kien Ly 2 , Karine M. David 2 , Richard C. Gardner 2 , Bruno Fedrizzi 1
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2016-12-22 00:00:00 , DOI: 10.1021/acschembio.6b00815
Matias I. Kinzurik 1 , Kien Ly 2 , Karine M. David 2 , Richard C. Gardner 2 , Bruno Fedrizzi 1
Affiliation
|
During glycolysis, yeast generates methylglyoxal (MG), a toxic metabolite that affects growth. Detoxification can occur when glyoxylase I (GLO1) and glyoxylase II (GLO2) convert MG to lactic acid. We have identified an additional, previously unrecognized role for GLO1 in sulfur assimilation in the yeast Saccharomyces cerevisiae. During a screening for putative carbon–sulfur lyases, the glo1 deletion strain showed significant production of H2S during fermentation. The glo1 strain also assimilated sulfate inefficiently but grew normally on cysteine. These phenotypes are consistent with reduced activity of the O-acetyl homoserine sulfhydrylase, Met17p. Overexpression of Glo1p gave a dominant negative phenotype that mimicked the glo1 and met17 deletion strain phenotypes. Western analysis revealed reduced expression of Met17p in the glo1 deletion, but there was no indication of an altered conformation of Met17p or any direct interaction between the two proteins. Unravelling a novel function in sulfur assimilation and H2S generation in yeast for a gene never connected with this pathway provides new opportunities for the study of this molecule in cell signaling, as well as the potential regulation of its accumulation in the wine and beer industry.
中文翻译：
该GLO1基因是需要的全部活动Ø通过乙酰高丝氨酸硫化氢编码MET17
在糖酵解期间，酵母会产生甲基乙二醛（MG），这是一种会影响生长的有毒代谢产物。当乙醛酸酶I（GLO1）和乙醛酸酶II（GLO2）将MG转化为乳酸时，就会发生排毒。我们已经确定了GLO1在酿酒酵母中的硫同化中的另外一个以前未被认识的作用。在筛选假定的碳-硫裂解酶期间，glo1缺失菌株在发酵过程中显示出H 2 S的大量产生。该glo1该菌株也不能有效地吸收硫酸盐，但在半胱氨酸上正常生长。这些表型与O-乙酰基高丝氨酸巯基化酶Met17p活性降低一致。Glo1p的过表达给出了一个显性的负表型，该表型模仿了glo1和met17缺失菌株的表型。Western分析显示，glo1缺失中Met17p的表达减少，但没有迹象表明Met17p的构象发生改变或两种蛋白之间存在任何直接相互作用。揭示硫同化和H 2的新功能从未与该途径相关联的基因在酵母中产生S为在细胞信号转导中研究该分子以及在葡萄酒和啤酒行业中对其积累的潜在调控提供了新的机会。
更新日期：2016-12-22
中文翻译：
该GLO1基因是需要的全部活动Ø通过乙酰高丝氨酸硫化氢编码MET17
在糖酵解期间，酵母会产生甲基乙二醛（MG），这是一种会影响生长的有毒代谢产物。当乙醛酸酶I（GLO1）和乙醛酸酶II（GLO2）将MG转化为乳酸时，就会发生排毒。我们已经确定了GLO1在酿酒酵母中的硫同化中的另外一个以前未被认识的作用。在筛选假定的碳-硫裂解酶期间，glo1缺失菌株在发酵过程中显示出H 2 S的大量产生。该glo1该菌株也不能有效地吸收硫酸盐，但在半胱氨酸上正常生长。这些表型与O-乙酰基高丝氨酸巯基化酶Met17p活性降低一致。Glo1p的过表达给出了一个显性的负表型，该表型模仿了glo1和met17缺失菌株的表型。Western分析显示，glo1缺失中Met17p的表达减少，但没有迹象表明Met17p的构象发生改变或两种蛋白之间存在任何直接相互作用。揭示硫同化和H 2的新功能从未与该途径相关联的基因在酵母中产生S为在细胞信号转导中研究该分子以及在葡萄酒和啤酒行业中对其积累的潜在调控提供了新的机会。
京公网安备 11010802027423号