Cell-Penetrating Cross-β Peptide Assemblies with Controlled Biodegradable Properties,Biomacromolecules

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Cell-Penetrating Cross-β Peptide Assemblies with Controlled Biodegradable Properties
Biomacromolecules ( IF 5.5 ) Pub Date : 2016-12-21 00:00:00 , DOI: 10.1021/acs.biomac.6b01153
Sanghun Han ₁ , Mun-kyung Lee ₁ , Yong-beom Lim ₁

Affiliation

Although self-assembled peptide nanostructures (SPNs) have shown potential as promising biomaterials, there is a potential problem associated with the extremely slow hydrolysis rate of amide bonds. Here, we report the development of cell-penetrating cross-β SPNs with a controllable biodegradation rate. The designed self-assembling β-sheet peptide incorporating a hydrolyzable ester bond (self-assembling depsipeptide; SADP) can be assembled into bilayer β-sandwich one-dimensional (1D) fibers similarly to conventional β-sheet peptides. The rate of hydrolysis can be controlled by the pH, temperature, and structural characteristics of the ester unit. The 1D fiber of the SADP transforms into vesicle-like 3D structures when the hydrophilic cell-penetrating peptide segment is attached to the SADP segment. Efficient cell internalization of the 3D nanostructures was observed, and we verified the intracellular degradation and disassembly of the biodegradable nanostructures. This study illustrates the potential of biodegradable cross-β SPNs and provides a valuable toolkit that can be used with self-assembling peptides.

中文翻译：

具有可控制的可生物降解特性的可穿透细胞的交叉β肽组件

尽管自组装的肽纳米结构（SPNs）已显示出作为有前途的生物材料的潜力，但存在与酰胺键极慢的水解速度相关的潜在问题。在这里，我们报道了具有可控制的生物降解速率的穿透细胞的交叉βSPNs的发展。与常规β-折叠肽相似，设计的并入有可水解酯键的自组装β-折叠肽（自组装双缩肽； SADP）可以组装成双层β-夹心一维（1D）纤维。水解速率可以通过pH，温度和酯单元的结构特征来控制。当亲水性细胞穿透肽段连接到SADP段时，SADP的1D纤维会转变为囊状3D结构。观察到3D纳米结构的高效细胞内在化，并且我们验证了可生物降解纳米结构的细胞内降解和分解。这项研究说明了可生物降解的交叉βSPN的潜力，并提供了可与自组装肽一起使用的有价值的工具包。

更新日期：2016-12-21

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