ATP analogues have been powerful compounds for the study of kinase‐catalyzed phosphorylation. However, the cell impermeability of ATP analogues has largely limited their use to in vitro lysate‐based experiments. Herein, we report the first cell‐permeable ATP analogue, ATP–polyamine–biotin (APB). APB is shown to promote biotin labeling of kinase substrates in live cells and has future applications in phosphoprotein purification and analysis. More generally, these studies provide a foundation for the development of additional cell‐permeable ATP analogues for cell‐signaling research.

中文翻译：

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细胞渗透性ATP类似物用于激酶催化的生物素化

ATP类似物是用于激酶催化的磷酸化研究的强大化合物。但是，ATP类似物的细胞不渗透性极大地限制了它们在体外基于裂解物的实验中的使用。在这里，我们报道了第一个细胞可渗透的ATP类似物，ATP-多胺-生物素（APB）。已证明APB可以促进活细胞中激酶底物的生物素标记，并在磷蛋白的纯化和分析方面具有未来的应用。更一般而言，这些研究为开发其他细胞渗透性ATP类似物用于细胞信号研究提供了基础。