Our aim was to determine the quantitative effects of a single-dose of Nattokinase (NK) administration on coagulation/fibrinolysis parameters comprehensively in healthy male subjects. A double-blind, placebo-controlled cross-over NK intervention study was carried out in 12 healthy young males. Following the baseline blood draw, each subject was randomized to receive either a single-dose of 2,000 FU NK (NSK-SD, Japan Bio Science Laboratory Co., Ltd) or placebo with subsequent cross-over of the groups. Subjects donated blood samples at 2, 4, 6 and 8 hours following administration for analysis of coagulation/fibrinolysis parameters. As a result, D-dimer concentrations at 6, and 8 hours, and blood fibrin/fibrinogen degradation products at 4 hours after NK administration elevated significantly (p < 0.05, respectively). Factor VIII activity declined at 4 and 6 hours (p < 0.05, respectively), blood antithrombin concentration was higher at 2 and 4 hours (p < 0.05, respectively), and the activated partial thromboplastin time prolonged significantly at 2 and 4 hours following NK administration (p < 0.05 and p < 0.01, respectively). All the changes, however, were within the normal range. In conclusion, thus, a single-dose of NK administration appears enhancing fibrinolysis and anti-coagulation via several different pathways simultaneously.

中文翻译：

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口服纳豆激酶单剂量可增强溶栓和抗凝特性。

我们的目标是全面确定健康男性受试者中单剂量纳豆激酶（NK）给药对凝血/纤维蛋白溶解参数的定量影响。在12位健康的年轻男性中进行了双盲，安慰剂对照的NK交叉干预研究。在基线抽血之后，将每个受试者随机接受单剂量的2,000 FU NK（NSK-SD，日本生物科学实验室有限公司）或安慰剂，随后进行分组。受试者在给药后第2、4、6和8小时捐献血液样品，以分析凝血/纤维蛋白溶解参数。结果，NK给药后第6、8小时的D-二聚体浓度和第4小时的血纤维蛋白/纤维蛋白原降解产物显着升高（分别为p <0.05）。凝血因子VIII的活性在4和6小时下降（分别为p <0.05），血液抗凝血酶浓度在2和4小时更高（分别为p <0.05），并且活化的部分凝血活酶时间在NK后2和4小时显着延长给药（分别为p <0.05和p <0.01）。但是，所有更改都在正常范围内。总之，因此，单剂量NK给药似乎同时通过几种不同途径增强了纤维蛋白溶解和抗凝作用。在正常范围内。总之，因此，单剂量NK给药似乎同时通过几种不同途径增强了纤维蛋白溶解和抗凝作用。在正常范围内。总之，因此，单剂量NK给药似乎同时通过几种不同途径增强了纤维蛋白溶解和抗凝作用。