血管内皮移植组织工程改进内皮细胞化的周细胞种子双肽支架。,Advanced Healthcare Materials

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血管内皮移植组织工程改进内皮细胞化的周细胞种子双肽支架。
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2016-10-26 , DOI: 10.1002/adhm.201600699
Paola Campagnolo ₁ , Adam J. Gormley ₁ , Lesley W. Chow ₁ , Anne Géraldine Guex _{1,

2} , Paresh A. Parmar ₁ , Jennifer L. Puetzer ₁ , Joseph A. M. Steele ₁ , Alexandre Breant ₁ , Paolo Madeddu ₃ , Molly M. Stevens ₁

Affiliation

用于冠状动脉旁路的合成血管移植物的开发受到内皮化不足（增加血栓形成的风险）和缺乏天然细胞成分（有利于病理重塑）的挑战。在这里，提出了一种具有合成血管移植应用潜力的双功能电纺聚（ε-己内酯）（PCL）支架。该支架包含两个束缚肽：“管腔”侧的骨桥蛋白衍生肽（Adh）和“管腔”侧的肝素结合肽（Hep）。此外，支架的“腔底”侧接种了隐静脉衍生的周细胞（SVPs），作为促血管生成生长因子的来源。Adh肽可显着增加内皮细胞的黏附力，而Hep肽促进SVP分泌的血管内皮生长因子的积累。在Transwell分析和在PCL支架上进行的改良刮擦分析中，SVP均可增加内皮细胞的迁移。SVPs植入支架的“ abluminal / Hep”一侧会进一步增加内皮细胞密度，表明这些肽和周细胞具有联合作用。最后，通过在无异种培养基中冷冻来保存接种SVP的支架，从而保持良好的细胞活力和功能。总之，这种工程支架结合了患者衍生的周细胞和空间组织的功能，可协同提高内皮细胞密度和生长因子保留率。SVPs植入支架的“ abluminal / Hep”一侧会进一步增加内皮细胞密度，表明这些肽和周细胞具有联合作用。最后，通过在无异种培养基中冷冻来保存接种SVP的支架，从而保持良好的细胞活力和功能。总之，这种工程支架结合了患者来源的周细胞和空间组织的功能，可协同提高内皮细胞密度和生长因子保留率。SVPs植入支架的“ abluminal / Hep”一侧会进一步增加内皮细胞密度，表明这些肽和周细胞具有联合作用。最后，通过在无异种培养基中冷冻来保存接种SVP的支架，从而保持良好的细胞活力和功能。总之，这种工程支架结合了患者衍生的周细胞和空间组织的功能，可协同提高内皮细胞密度和生长因子保留率。

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Pericyte Seeded Dual Peptide Scaffold with Improved Endothelialization for Vascular Graft Tissue Engineering

The development of synthetic vascular grafts for coronary artery bypass is challenged by insufficient endothelialization, which increases the risk of thrombosis, and the lack of native cellular constituents, which favors pathological remodeling. Here, a bifunctional electrospun poly(ε‐caprolactone) (PCL) scaffold with potential for synthetic vascular graft applications is presented. This scaffold incorporates two tethered peptides: the osteopontin‐derived peptide (Adh) on the “luminal” side and a heparin‐binding peptide (Hep) on the “abluminal” side. Additionally, the “abluminal” side of the scaffold is seeded with saphenous vein‐derived pericytes (SVPs) as a source of proangiogenic growth factors. The Adh peptide significantly increases endothelial cell adhesion, while the Hep peptide promotes accumulation of vascular endothelial growth factor secreted by SVPs. SVPs increase endothelial migration both in a transwell assay and a modified scratch assay performed on the PCL scaffold. Seeding of SVPs on the “abluminal”/Hep side of the scaffold further increases endothelial cell density, indicating a combinatory effect of the peptides and pericytes. Finally, SVP‐seeded scaffolds are preserved by freezing in a xeno‐free medium, maintaining good cell viability and function. In conclusion, this engineered scaffold combines patient‐derived pericytes and spatially organized functionalities, which synergistically increase endothelial cell density and growth factor retention.

更新日期：2016-10-26

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