1-Amino-2,2-difluorocyclopropane-1-carboxylic acid (DFACC) is of interest in the study of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase due to the increased reactivity of its cyclopropyl functionality. It is shown that DFACC is unstable under near-physiological conditions where it primarily decomposes via specific-base catalysis to 3-fluoro-2-oxobut-3-enoic acid with a rate constant of 0.18 ± 0.01 min^–1. Upon incubation with ACC deaminase, DFACC is found to be a slow-dissociating inhibitor of ACC deaminase with submicromolar affinity.

中文翻译：

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1-氨基-2,2-二氟环丙烷-1-羧酸的研究：分解和抑制1-氨基环丙烷-1-羧酸脱氨酶的机理

1-氨基-2,2-二氟环丙烷-1-羧酸（DFACC）因其环丙基官能团反应性的提高而在1-氨基环丙烷-1-羧酸（ACC）脱氨酶的研究中受到关注。结果表明，DFACC在近乎生理条件下不稳定，在该条件下，它主要通过特异性碱催化分解为3-氟-2-氧代丁氧3-烯酸，速率常数为0.18±0.01 min ^–1。与ACC脱氨酶一起孵育后，发现DFACC是具有亚微摩尔亲和力的ACC脱氨酶的缓慢解离抑制剂。