New miniaturised microfluidic biofilter (BF) devices based on silicon micropillars have been developed and tested regarding their ability to extract HBV (Hepatitis B Virus) bacterial DNA from biological sample solutions. The device is composed of a silicon microchannel in which the pillars are distributed at the bottom surface. The extracted DNA solutions were analysed by real time PCR amplification and the biofilter performance was evaluated. The results obtained show that the DNA binding to the biofilters and the elution efficiency strictly depend on the pillars’ geometrical dimensions and increase proportionally with the surface/volume ratio. The device exhibiting the best extraction efficiency was then tested in combination with a silicon integrated real time PCR amplification chip as a preliminary step towards the development of genetic point-of-care devices.

中文翻译：

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基于硅微柱的新型微型生物滤池用于核酸提取

基于硅微柱的新型微型微流控生物过滤器（BF）装置已经开发并经过测试，能够从生物样品溶液中提取HBV（乙型肝炎病毒）细菌DNA。该器件由硅微通道组成，其中的支柱分布在底表面。提取的DNA溶液通过实时PCR扩增进行分析，并评估了生物滤池的性能。获得的结果表明，DNA与生物滤池的结合和洗脱效率严格取决于柱的几何尺寸，并随表面/体积比成比例增加。