Using a dual DNA aptamer (CEA aptamer linked to hemin aptamer), capable of rapidly capturing carcinoembryonic antigen (CEA) and hemin, an all-in-one dual-aptasensor with 1,1′-oxalyldiimidazole (ODI) chemiluminescence detection was developed for the early diagnosis of human cancer. CEA and hemin competitively bound with the dual DNA aptamer while the mixture in a detection cell was incubated for 30min at room temperature. When Amplex Red and H₂O₂ were added in the detection cell after the incubation, the yield of resorufin formed from the reaction Amplex Red and H₂O₂ depended on the concentration of HRP-mimicking G-quardruplex DNAzyme formed from the binding interaction between hemin and the dual DNA aptamer. Bright red light was observed with the addition of ODI and H₂O₂ in the detection cell containing resorufin. Relative CL intensity of all-in-one dual-aptasensor, operated with the competitive reaction of CEA and hemin in the presence of the dual aptamer, was exponentially decreased with the increase of CEA concentration in human serum. The limit of detection (LOD=3σ) of the all-in-one dual-aptasensor which operated with excellent accuracy, precision, and reproducibility was as low as 0.58ng/ml. The good correlation between the easy to use all-in-one dual-aptasensor and conventional enzyme-linked immunosorbent assay (ELISA), operated with time consuming procedures (e.g., long incubations and multiple washings), indicates that the rapid all-in-one dual-aptasensor can be applied as a novel clinical tool for the early diagnosis of breast cancer.

中文翻译：

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能够快速定量癌胚抗原的多合一双适体传感器

使用能够快速捕获癌胚抗原（CEA）和血红素的双DNA适体（与Hemin适体连接的CEA适体），开发了一种具有1,1'-草酰二咪唑（ODI）化学发光检测的多合一双适体传感器。人类癌症的早期诊断。CEA和血红素与双DNA适体竞争性结合，而检测细胞中的混合物在室温下孵育30分钟。孵育后在检测池中添加Amplex Red和H ₂ O _2时，由Amplex Red和H ₂ O ₂反应形成的试卤灵的产量。依赖于由血红素和双重DNA适体之间的结合相互作用形成的模仿HRP的G-四链体DNA核酶的浓度。加入ODI和H ₂ O ₂观察到亮红色的光在含有试卤灵的检测池中。随着人血清中CEA浓度的增加，在CEA和血红素在双重适体存在下的竞争反应下运行的多合一双重适体传感器的相对CL强度呈指数下降。具有出色的准确性，精密度和可重复性的多合一双适体传感器的检出限（LOD =3σ）低至0.58ng / ml。易于使用的多合一双适体传感器与常规的酶联免疫吸附测定（ELISA）（采用耗时的程序（例如，长时间孵育和多次清洗））之间的良好相关性表明，快速的全合一一个双适体传感器可以用作乳腺癌的早期诊断的新型临床工具。